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PF-4708671

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Catalog No. T2002Cas No. 1255517-76-0
Alias PF4708671

PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM).

PF-4708671

PF-4708671

🥰Excellent
Purity: 99.67%
Catalog No. T2002Alias PF4708671Cas No. 1255517-76-0
PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM).
Pack SizePriceAvailabilityQuantity
5 mg$47In Stock
10 mg$70In Stock
25 mg$113In Stock
50 mg$153In Stock
100 mg$279In Stock
1 mL x 10 mM (in DMSO)$77In Stock
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Purity:99.67%
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Product Introduction

Bioactivity
Description
PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM).
Targets&IC50
p70 S6K1:160 nM
In vivo
PF-4708671 inhibits the phosphorylation of S6 protein mediated by S6K1 in response to IGF-1 (Insulin-like Growth Factor 1) and can also induce the phosphorylation of the T-loop and hydrophobic motif of S6K1 through mTORC1 (mTOR complex 1).
Kinase Assay
Affinity determination: Purified activated FAK kinase domain (amino acids 410–689) is reacted with 50 μM ATP, and 10 μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly(Glu/Tyr) in kinase buffer (50 mM HEPES, pH 7.5, 125 mM NaCl, 48 mM MgCl2) for 15 min. Phosphorylation of poly(Glu/Tyr) is challenged with serially diluted compounds at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is run in triplicate. Phosphorylation of poly(Glu/Tyr) is detected with a general anti-phospho-tyrosine (PY20) antibody, followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. The standard horseradish peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine is added, and Optical Density readings at 450 nm are obtained following the addition of stop solution (2 M H2SO4). The IC50 values are determined using the Hill slope model.
AliasPF4708671
Chemical Properties
Molecular Weight390.41
FormulaC19H21F3N6
Cas No.1255517-76-0
SmilesCCc1cncnc1N1CCN(Cc2nc3cc(ccc3[nH]2)C(F)(F)F)CC1
Relative Density.1.348 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 19.5 mg/mL (50 mM)
Ethanol: 19.5 mg/mL (50 mM)
Solution Preparation Table
DMSO/Ethanol
1mg5mg10mg50mg
1 mM2.5614 mL12.8070 mL25.6141 mL128.0705 mL
5 mM0.5123 mL2.5614 mL5.1228 mL25.6141 mL
10 mM0.2561 mL1.2807 mL2.5614 mL12.8070 mL
20 mM0.1281 mL0.6404 mL1.2807 mL6.4035 mL
50 mM0.0512 mL0.2561 mL0.5123 mL2.5614 mL

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