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1400W dihydrochloride

Catalog No. T3491 CAS 214358-33-5

Synonyms: 1400W 2HCl, N-(3-(Aminomethyl)benzyl)acetamidine

1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

1400W dihydrochloride Chemical Structure

1400W dihydrochloride, CAS 214358-33-5

Pack Size	Availability	Price/USD
5 mg	In stock	$ 43.00
10 mg	In stock	$ 68.00
25 mg	In stock	$ 127.00
50 mg	In stock	$ 231.00
100 mg	In stock	$ 393.00
200 mg	In stock	$ 511.00
500 mg	In stock	$ 829.00
1 mL * 10 mM (in DMSO)	In stock	$ 48.00

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Purity: 100%

Purity: 99.47%

Purity: 99.31%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).
Targets&IC50	nNOS:2 μM(Ki), iNOS:<7 nM(Kd), eNOS:50 μM(Ki)
In vitro	1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].
In vivo	1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].
Cell Research	RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody. (Only for Reference)

Synonyms	1400W 2HCl, N-(3-(Aminomethyl)benzyl)acetamidine
Molecular Weight	250.17
Formula	C10H17Cl2N3
CAS No.	214358-33-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 139.9 mM

DMSO: 139.9 mM

References and Literature

1. Garvey EP, et al. J Biol Chem. 199, 272(8):4959-63. 2. Ersoz N, et al. Ren Fail. 2009, 31(8):704-10. 3. Sen N, et al. Nat Cell Biol. 2008, 10(7):866-73. 4. Thomsen LL, et al. Cancer Res. 1997, 57(15):3300-4.

Citations

1. Cai J, Zhang X, Chen P, et al. The ER stress sensor inositol-requiring enzyme 1α in Kupffer cells promotes hepatic ischemia-reperfusion injury. Journal of Biological Chemistry. 2021: 101532.

Related compound libraries

This product is contained In the following compound libraries：

Highly Selective Inhibitor Library Inhibitor Library Bioactive Compounds Library Max Human Metabolite Library NO PAINS Compound Library Immunology/Inflammation Compound Library Anti-Aging Compound Library Nonsteroidal Anti-Inflammatory Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

1400W dihydrochloride 214358-33-5 Immunology/Inflammation NOS NO Synthase inhibit 1400W 2HCl N-(3-(Aminomethyl)benzyl)acetamidine Nitric oxide synthases 1400W Dihydrochloride 1400W Inhibitor inhibitor

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