Powder: -20°C for 3 years | In solvent: -80°C for 1 year
3-Deazaadenosine hydrochloride is an inhibitor of S-adenosylhomocysteine hydrolase (Ki: 3.9 μM). It has anti-inflammatory, anti-proliferative, and anti-HIV activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 128.00 | |
5 mg | In stock | $ 228.00 | |
10 mg | In stock | $ 393.00 | |
25 mg | In stock | $ 652.00 | |
50 mg | In stock | $ 913.00 | |
100 mg | In stock | $ 1,230.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 152.00 |
Description | 3-Deazaadenosine hydrochloride is an inhibitor of S-adenosylhomocysteine hydrolase (Ki: 3.9 μM). It has anti-inflammatory, anti-proliferative, and anti-HIV activity. |
Targets&IC50 | HIV-1, A018 isolate:0.20 µM , HIV-1, A012 isolate:0.15, S-adenosylhomocysteine hydrolase:(ki)3.9 µM |
In vitro | 3-Deazaadenosine shows the anti-HIV effect and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018, IC50s: 0.15 and 0.20 μM) [1]. 3-Deazaadenosine (1-100 μM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances the nuclear translocation of NF-κB but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine [2]. 3-Deazaadenosine (50, 100 μM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 μM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling [3]. |
Cell Research | The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 10^5 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 10^5 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA [1]. |
Molecular Weight | 302.71 |
Formula | C11H15ClN4O4 |
CAS No. | 86583-19-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40.67 mg/mL(134.35 mM)
H2O: 20 mg/mL
You can also refer to dose conversion for different animals. More
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3-Deazaadenosine hydrochloride 86583-19-9 Microbiology/Virology Others Proteases/Proteasome HIV Protease HIV 3-Deazaadenosine Human immunodeficiency virus Inhibitor 3-Deazaadenosine Hydrochloride 3 Deazaadenosine hydrochloride 3Deazaadenosine hydrochloride inhibit inhibitor