Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

AZD7545

AZD7545
AZD7545 is a potent PDHK inhibitor.
Catalog No. T2447Cas No. 252017-04-2
Select Batch
Purity:99.13%
Contact us for more batch information

Resource Download

AZD7545

Purity: 99.13%
Catalog No. T2447Cas No. 252017-04-2

AZD7545 is a potent PDHK inhibitor.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 mg$40In Stock
5 mg$92In Stock
10 mg$139In Stock
25 mg$239In Stock
50 mg$372In Stock
100 mg$513In Stock
1 mL x 10 mM (in DMSO)$97In Stock
Bulk & Custom
Add to Cart
Questions
View More

Related Compound Libraries of "AZD7545"

Product Introduction

Bioactivity
Description
AZD7545 is a potent PDHK inhibitor.
Targets&IC50
PDHK2:6.4 nM, PDHK1:36.8 nM
In vitro
In obese, insulin-resistant Zucker rats, AZD7545 eliminated postprandial hyperglycemia. AZD7545 increased the percentage of active PDH in the liver and skeletal muscle of Wistar rats.
In vivo
AZD7545 inhibits pyruvate dehydrogenase kinase (PDHK) activity by disrupting the interaction between the inner lipoic acid domain of PDHK2 and the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex (PDC). It increases the activity of pyruvate dehydrogenase (PDH) in primary rat hepatocytes with an EC50 of 105 nM. In the presence of recombinant human PDHK2, AZD7545 also enhances PDH activity, achieving an EC50 of 5.2 nM.
Kinase Assay
Cellular Kinase Assays: NIH3T3 cells prepared by transfection of human KDR. The cells are cultured in a collagen type I coated 96-well plate in an amount of 1.5 × 104 per well. The medium is then replaced by a DMEM medium containing 0.1% FCS. Ki8751 diluted in DMSO is added to each well and cultured. rhVEGF is added to a final concentration of 100 ng/mL, and the stimulation of cells is carried out at 37 °C. The cells are washed with PBS (pH 7.4), 50 μL of a solubilization buffer (20 mM HEPES (pH 7.4), 150 mM NaCl, 0.2% Triton X-100, 10% glycerol, 5 mM Na3VO4, 5 mM disodium ethylenediamine tetraacetate, and 2 mM Na4P2O7) is then added and a cell extract is prepared. Separately, PBS (50 μL, pH 7.4) containing 5 μg/mL of antiphosphotyrosine antibody (PY20) is added to a microplate for ELISA. After washing of the plate, 300 μL of a blocking solution is added. The cell extract is transferred to the plate. An anti-VEGFR2 antibody and a peroxidase-labeled anti-rabbit Ig antibody are added. Next, a chromophoric substrate for peroxidase is added, and the absorbance at 450 nm is measured with microplate reader. The VEGFR2 phosphorylation activity for each well is determined by presuming the absorbance with the addition of VEGF and without the addition of the test sample to be 100% VEGFR2 phosphorylation activity and VEGF to be 0% VEGFR2 phosphorylation activity. The concentration of the inhibition (%) of VEGFR2 Phosphorylation is determined for each case, and IC50 value is calculated.
Chemical Properties
Molecular Weight478.87
FormulaC19H18ClF3N2O5S
Cas No.252017-04-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 88 mg/mL (183.8 mM)
DMSO: 88 mg/mL (183.8 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.0882 mL10.4412 mL20.8825 mL104.4125 mL
5 mM0.4176 mL2.0882 mL4.1765 mL20.8825 mL
10 mM0.2088 mL1.0441 mL2.0882 mL10.4412 mL
20 mM0.1044 mL0.5221 mL1.0441 mL5.2206 mL
50 mM0.0418 mL0.2088 mL0.4176 mL2.0882 mL
100 mM0.0209 mL0.1044 mL0.2088 mL1.0441 mL

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
%Tween 80
%ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy AZD7545 | purchase AZD7545 | AZD7545 cost | order AZD7545 | AZD7545 chemical structure | AZD7545 in vivo | AZD7545 in vitro | AZD7545 formula | AZD7545 molecular weight