Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Bay 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 30.00 | |
5 mg | In stock | $ 43.00 | |
10 mg | In stock | $ 61.00 | |
25 mg | In stock | $ 77.00 | |
50 mg | In stock | $ 126.00 | |
100 mg | In stock | $ 207.00 | |
200 mg | In stock | $ 269.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 56.00 |
Description | Bay 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM). |
Targets&IC50 | IκBα phosphorylation:10 μM |
Kinase Assay | In Gel Kinase Assay: In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30?°C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control. |
Cell Research | Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.(Only for Reference) |
Synonyms | BAY 11-7083 |
Molecular Weight | 249.33 |
Formula | C13H15NO2S |
CAS No. | 196309-76-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 24.9 mg/mL (100 mM)
Ethanol: 24.9 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Bay 11-7085 196309-76-9 Apoptosis NF-Κb IκB/IKK Ferroptosis NF-κB BAY 11-7085 BAY 11-7083 inhibit Nuclear factor-κB Inhibitor Bay 11 7085 Bay 117085 Nuclear factor-kappaB inhibitor