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Dimethyl fumarate

Catalog No. T0492 CAS 624-49-7

Synonyms: DMF

Dimethyl fumarate (DMF), a fumarate derivative, is used as antiinflammatory and immunomodulatory agent.

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Dimethyl fumarate, CAS 624-49-7

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500 mg	In stock	$ 45.00

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Purity: 90.51%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Dimethyl fumarate (DMF), a fumarate derivative, is used as antiinflammatory and immunomodulatory agent.
In vitro	PDE activity is determined with some modifications. The assay mixture contain 50 mM Tris (pH 7.4), 5 mM MgCl2, 0.5 μM cAMP or cGMP, and [3H]cAMP or [3H]cGMP (about 30,000 cpm/assay), the indicated concentration of the inhibitor and an aliquot of the enzyme solution at a final assay volume of 200 μL. Stock solutions of the compounds are diluted 1:100 (v/v) in the Tris buffer mentioned above; appropriate dilutions are prepared in 1% (v/v) DMSO/Tris buffer, which are diluted 1:2 (v/v) in the assays to obtain the desired final concentrations of the inhibitors at a DMSO concentration of 0.5% (v/v). DMSO itself affected none of the PDE activities. After preincubation for 5 min at 37°C, the reaction is started by the addition of substrate (cAMP or cGMP) and the assays are incubated for further 15 min at 37°C. Then 50 μL of 0.2 N HCl is added to stop the reaction and the assays are left on ice for about 10 min. Following incubation with 25 μg of 5′-nucleotidase (Crotalus atrox snake venom) for 10 min at 37°C, the assays are loaded on QAE Sephadex A-25 (1 mL of bed volume in Poly-Prep chromatography columns). The columns are eluted with 2 mL of 30 mM ammonium formate (pH 6.0) and the eluate is counted for radioactivity. Results are corrected for blank values (measured in the presence of denatured protein) that are below 5% of total radioactivity. The amount of cyclic nucleotides hydrolyzed did not exceed 30% of the original substrate concentration[3].
In vivo	Dimethyl fumarate inhibits nuclear entry of NF-kappaB in RHEC and reduces myocardial infarct size after ischemia and reperfusion in rats in vivo. [3] Dimethyl fumarate oral administration is shown to upregulate mRNA and protein levels of Nrf2 and Nrf2-regulated cytoprotective genes, attenuate 6-OHDA induced striatal oxidative stress and inflammation in C57BL/6 mice. [5]

Synonyms	DMF
Molecular Weight	144.13
Formula	C6H8O4
CAS No.	624-49-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 14.4 mg/mL (100 mM)

Ethanol: 7.2 mg/mL (50 mM)

References and Literature

1. Albrecht P, et al. J Neuroinflammation,2012, 9, 163. 2. Peng H, et al. J Biol Chem,2012, 287(33), 282017-28026. 3. Meili-Butz S, et al. Eur J Pharmacol,2008, 586(1-3), 251-258. 4. Cross SA, et al. J Immunol,2011, 187(10), 52015-5025. 5. Jing X, et al. Neuroscience,2015, 286, 131-140. 6. Li Y, et al. Dimethyl fumarate accelerates wound healing under diabetic condition. J Mol Endocrinol. 2018 Jul 23. pii: JME-18-0102. 7. Wang Y, Ma H, Huang J, et al. Discovery of bardoxolone derivatives as novel orally active necroptosis inhibitors[J]. European Journal of Medicinal Chemistry. 2020: 113030.

Citations

1. Wang Y, Ma H, Huang J, et al. Discovery of bardoxolone derivatives as novel orally active necroptosis inhibitors. European Journal of Medicinal Chemistry,. 2021 Feb 15;212:113030

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library EMA Approved Drug Library Anti-Neurodegenerative Disease Compound Library Drug-Fragment Library NO PAINS Compound Library Bioactive Compounds Library Max Covalent Inhibitor Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Dimethyl fumarate 624-49-7 Autophagy Immunology/Inflammation Metabolism Microbiology/Virology NF-Κb Proteases/Proteasome Reactive Oxygen Species Nrf2 Endogenous Metabolite HIV Protease Antioxidant neuroprotective Dimethyl Inhibitor Keap1-Nrf2 inhibit anti-inflammatory DMF Dimethyl Fumarate brain-penetrant ROS Human immunodeficiency virus sclerosis HIV inhibitor

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