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Disulfiram

Catalog No. T0054 CAS 97-77-8

Synonyms: Tetraethylthiuram disulfide, NSC 190940, TETD

Disulfiram (TETD) is an inhibitor of the acetaldehyde dehydrogenase ALDH, inhibiting hALDH1 and hALDH2 (IC50=0.15/1.45 μM) with specificity. Disulfiram is acutely sensitized to alcohol and also inhibits GSDMD pore formation.

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Disulfiram, CAS 97-77-8

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1 g	In stock	$ 50.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.79%

Purity: 95%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Disulfiram (TETD) is an inhibitor of the acetaldehyde dehydrogenase ALDH, inhibiting hALDH1 and hALDH2 (IC50=0.15/1.45 μM) with specificity. Disulfiram is acutely sensitized to alcohol and also inhibits GSDMD pore formation.
In vitro	Disulfiram inhibits the P-glycoprotein efflux pump, suppresses the transcription factor NF-κB (nuclear factor-κB), reduces angiogenesis, and inhibits tumor growth in mice. Its antitumor activity is associated with in vivo protease inhibition. Disulfiram also induces apoptosis. In severe combined immunodeficient mice with melanoma xenografts, it impedes growth and angiogenesis, an effect that is enhanced by zinc supplementation.
In vivo	Disulfiram, clinically employed as an anti-alcoholism agent, potently inhibits both constitutive and 5-FU-induced NF-κB activity in a dose-dependent manner. In the DLD-1 and RKO (WT) cell lines, Disulfiram significantly augments the apoptotic effects of 5-FU and synergistically enhances its cytotoxicity. In melanoma cells, co-treatment with copper or zinc ions reduces cyclin A expression compared to Disulfiram alone and inhibits proliferation in vitro. Moreover, Disulfiram decreases viable cell count, with copper chloride addition markedly intensifying DSF-induced cell death.
Cell Research	The effect of disulfiram (0.15-5.0 μM) or sodium diethyldithiocarbamate (1.0 μM) on proliferation of malignant cell lines is studied in cultures stimulated with 10% FBS. Cell numbers are quantitated 24 to 72 hours later, as outlined below. In some experiments, disulfiram is added immediately after cells are plated. In other experiments, cells are plated and allowed to grow for 24 to 72 hours before fresh medium with disulfiram is added and cell numbers are assayed 24 to 72 hours later. Synergy is studied between disulfiram and?N,N′-bis(2-chloroethyl-N-nitrosourea (carmustine, 1.0-1,000 μM) or cisplatin (0.1-100 μg/mL) added to medium. The effect of metal ions on disulfiram is studied with 0.2 to 10 μM Cu2+?(provided as CuSO4), Zn2+?(as ZnCl2), Ag+?(as silver lactate), or Au3+?(as HAuCl4·3Water) ions added to growth medium, buffered to physiologic pH. To provide a biologically relevant source of copper, medium is supplemented with human ceruloplasmin at doses replicating low and high normal adult serum concentrations (250 and 500 mg/mL).

Synonyms	Tetraethylthiuram disulfide, NSC 190940, TETD
Molecular Weight	296.54
Formula	C10H20N2S4
CAS No.	97-77-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 29.7 mg/mL (100 mM)

Ethanol: 29.7 mg/mL (100 mM)

References and Literature

1. Nasrollahzadeh A, et al. Anti-proliferative activity of disulfiram through regulation of the AKT-FOXO axis: A proteomic study of molecular targets. Biochim Biophys Acta Mol Cell Res. 2021 Sep;1868(10):119087. 2. Wang Q, et al. Disulfiram bolsters T-cell anti-tumor immunity through direct activation of LCK-mediated TCR signaling. EMBO J. 2022 Aug 16;41(16):e110636. 3. Saitoh A, et al. Disulfiram Produces Potent Anxiolytic-Like Effects Without Benzodiazepine Anxiolytics-Related Adverse Effects in Mice. Front Pharmacol. 2022 Mar 7;13:826783. 4. Brar SS, et al. Mol Cancer Ther, 2004, 3(9), 1049-1060. 5. Bowden G D, Land K M, O'Connor R M, et al. High-throughput screen of drug repurposing library identifies inhibitors of Sarcocystis neurona growth[J]. International Journal for Parasitology: Drugs and Drug Resistance. 2018 Apr；8(1): 137-144. 6. Zeng X, Zhu S, Lu W, et al. Target identification among known drugs by deep learning from heterogeneous networks[J]. Chemical Science. 2020, 11(7): 1775-1797.

Citations

1. Zeng X, Zhu S, Lu W, et al. Target identification among known drugs by deep learning from heterogeneous networks. Chemical Science. 2020, 11(7): 1775-1797. 2. Yu S, Wang H, Liu M, et al. Loss of ATG5 in KRT14+ cells leads to accumulated functional impairments of salivary glands via pyroptosis. The FASEB Journal. 2022, 36(12): e22631. 3. Bowden G D, Land K M, O'Connor R M, et al. High-throughput screen of drug repurposing library identifies inhibitors of Sarcocystis neurona growth. International Journal for Parasitology: Drugs and Drug Resistance. 2018 Apr；8(1): 137-144 4. Yang Y, Zhu Y, Wang K, et al.Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy.Bioactive Materials.2023, 29: 116-131. 5. Cui W, Guo M, Liu D, et al.Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition.Nature Cell Biology.2024: 1-14. 6. Zhang Z D, Shi C R, Li F X, et al.Disulfiram ameliorates STING/MITA-dependent inflammation and autoimmunity by targeting RNF115.Cellular & Molecular Immunology.2024: 1-17. 7. Yuan X, Chen P, Luan X, et al.NLRP3 deficiency protects against acetaminophen‑induced liver injury by inhibiting hepatocyte pyroptosis.Molecular Medicine Reports.2024, 29(4): 1-15.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Inhibitor Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Apoptosis Compound Library Bioactive Compound Library Cysteine Covalent Library ReFRAME Related Library Anti-Viral Compound Library

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Keywords

Disulfiram 97-77-8 Apoptosis Immunology/Inflammation Metabolism Dehydrogenase Interleukin Pyroptosis inhibit Inhibitor Tetraethylthiuram Disulfide Aldehyde Dehydrogenase (ALDH) Tetraethylthiuram disulfide Interleukin Related NSC190940 NSC 190940 Tetraethylthiuram TETD NSC-190940 inhibitor

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