Home Tools

Doxycycline

Catalog No. T1687 CAS 564-25-0

Synonyms: Vibramycin, Doxiciclina, Doxytetracycline, Doxycyclinum

Doxycycline (Doxiciclina) belongs to the tetracycline class of antibiotics and is a broad-spectrum metalloproteinase (MMP) inhibitor with oral activity. Doxycycline has antibacterial activity and antitumor activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Doxycycline, CAS 564-25-0

Pack Size	Availability	Price/USD
25 mg	In stock	$ 45.00
50 mg	In stock	$ 72.00
100 mg	In stock	$ 97.00
200 mg	In stock	$ 133.00
500 mg	In stock	$ 174.00

Bulk Inquiry

Select Batch

Purity: 99.88%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Doxycycline (Doxiciclina) belongs to the tetracycline class of antibiotics and is a broad-spectrum metalloproteinase (MMP) inhibitor with oral activity. Doxycycline has antibacterial activity and antitumor activity.
In vitro	METHODS: Twenty-two tumor cells were treated with Doxycycline for 48 h. Cell viability was measured by MTT. RESULTS: Lung cancer cells were more sensitive to Doxycycline than most other cell lines. NCI-H446 and A549 cells were sensitive to Doxycycline with IC50s of 1.70 μM and 1.06 μM, respectively. [1] METHODS: Human pancreatic cancer cells PANC-1 were treated with Doxycycline (10-40 μg/mL) for 8-24 h. The cell cycle was detected by Flow Cytometry. RESULTS: Doxycycline caused the cells to arrest in the G1-S phase of the cell cycle. [2]
In vivo	METHODS: To detect anti-tumor activity in vivo, Doxycycline (50 mg/kg) was administered intraperitoneally once daily for fifteen days to BALB/c mice harboring human mammary carcinoma tumor 4T1. RESULTS: Doxycycline delayed tumor growth and reduced the number of tumor-associated macrophages and blood vessels. [3] METHODS: To assay antimicrobial activity in vivo, Doxycycline (40 mg/kg) was administered intraperitoneally to BALB/c mice exposed to B. pseudomallei 1026b twice daily for two weeks. RESULTS: Doxycycline-treated mice survived 100% of the time, and no B. pseudomallei DNA was amplified from the lungs or spleens of the majority of the surviving mice. [4]

Synonyms	Vibramycin, Doxiciclina, Doxytetracycline, Doxycyclinum
Molecular Weight	444.43
Formula	C22H24N2O8
CAS No.	564-25-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 84.44 mg/mL (190 mM), Sonification is recommended.

References and Literature

1. Qin Y, et al. Doxycycline reverses epithelial-to-mesenchymal transition and suppresses the proliferation and metastasis of lung cancer cells. Oncotarget. 2015 Dec 1;6(38):40667-79. 2. Son K, et al. Doxycycline induces apoptosis in PANC-1 pancreatic cancer cells. Anticancer Res. 2009 Oct;29(10):3995-4003. 3. Tang X, et al. Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation. Mol Cancer. 2017 Feb 8;16(1):36. 4. Gelhaus HC, et al. Efficacy of post exposure administration of doxycycline in a murine model of inhalational melioidosis. Sci Rep. 2013;3:1146.

Citations

1. Tan Y, Chen X, Xu Z, et al.The mechanics of tumor cells dictate malignancy via cytoskeleton-mediated APC/Wnt/β-catenin signaling.Research.2023 2. Li Y M, Mei Y C, Liu A H, et al.Gcn5-and Bre1-mediated Set2 degradation promotes chronological aging of Saccharomyces cerevisiae.Cell Reports.2023, 42(10). 3. Wang W, Wang M, Du T, et al.SHMT2 Promotes Gastric Cancer Development through Regulation of HIF1α/VEGF/STAT3 Signaling.International Journal of Molecular Sciences.2023, 24(8): 7150. 4. Lu J, Lu Z, Liu L, et al. Identification of crocin as a new hIAPP amyloid inhibitor via a simple yet highly biospecific screening system. Chemistry & Biodiversity. 2021 5. Xin Y, Chen X, Tang X, et al. Mechanics and actomyosin-dependent survival and chemoresistance of suspended tumor cells in shear flow. Biophysical Journal. 2019 May 21;116(10):1803-1814 6. Li Y, Qiao K, Zhang X, et al. Targeting myeloid-derived suppressor cells to attenuate vasculogenic mimicry and synergistically enhance the anti-tumor effect of PD-1 inhibitor. Iscience. 2021, 24(12): 103392. 7. Chen X, Fan Y, Sun J, et al. Nanoparticle-mediated specific elimination of soft cancer stem cells by targeting low cell stiffness. Acta Biomaterialia. 2021 8. Han L, Jiang Y, Shi M, et al.LIPH contributes to glycolytic phenotype in pancreatic ductal adenocarcinoma by activating LPA/LPAR axis and maintaining ALDOA stability.Journal of Translational Medicine.2023, 21(1): 1-21. 9. Hu Z, Lu Y, Cao J, et al.N-acetyltransferase NAT10 controls cell fates via connecting mRNA cytidine acetylation to chromatin signaling.Science Advances.2024, 10(2): eadh9871. 10. Wang J, Zhang B, Chen X, et al.Cell mechanics regulate the migration and invasion of hepatocellular carcinoma cells via JNK signaling.Acta Biomaterialia.2024

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Antiparasitic Natural Product Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Parasitic Compound Library Inhibitor Library Microbial Natural Product Library Anti-Cancer Active Compound Library Orally Active Compound Library

Related Products

Related compounds with same targets

Torilin TNF Protease Inhibitor 2 MMP-12 Inhibitor Glucosamine sulfate Apratastat BPHA BT1718 DB04760

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Doxycycline 564-25-0 Microbiology/Virology Proteases/Proteasome ribosome MMP Antibiotic Antibacterial Parasite Matrix metalloproteinases 293T MCF 12A antibacterial activity Vibramycin G55 Inhibitor Doxiciclina U343 LNT-229 Doxytetracycline anti-proliferative activity Bacterial Doxycyclinum inhibit SVG inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.