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JNK-IN-7

Catalog No. T3598   CAS 1408064-71-0
Synonyms: JNK inhibitor

JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.

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JNK-IN-7 Chemical Structure
JNK-IN-7, CAS 1408064-71-0
Pack Size Availability Price/USD Quantity
1 mg In stock $ 89.00
2 mg In stock $ 161.00
5 mg In stock $ 238.00
10 mg In stock $ 306.00
25 mg In stock $ 550.00
50 mg In stock $ 792.00
100 mg In stock $ 1,080.00
1 mL * 10 mM (in DMSO) In stock $ 258.00
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Purity: 99.58%
Purity: 99.53%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
Targets&IC50 JNK3:0.75 nM, JNK1:1.54 nM, JNK2:1.99nM
In vitro JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48?h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].
Kinase Assay A375 cells are pre-treated with 1 μM JNK-IN-7 for the indicated amounts of time. Remove the medium and wash 3 times with PBS. Resuspend the cell pellet with 1 mL Lysis Buffer (1% NP-40, 1% CHAPS, 25 mM Tris, 150 mM NaCl, Phosphatase Inhibitor Cocktail). Rotate end-to-end for 30 min at 4°C. Lysates are cleared by centrifugation at 14000 rpm for 15 min in the Eppendorf. The cleared lysates gel filtered into Kinase Buffer (0.1% NP-40, 20 mM HEPES, 150 mM NaCl, Phosphatase Inhibitor Cocktail, Protease Inhibitor Cocktail) using Bio-Rad 10DG colums. The total protein concentration of the gel-filtered lysate should be around 5-15 mg/mL. Cell lysate is labeled with the probe at 5 μM for 1 hour. Samples are reduced with DTT, and cysteines are blocked with iodoacetamide and gel filtered to remove excess reagents and exchange the buffer. Add 1 volume of 2X Binding Buffer (2% Triton-100, 1% NP-40, 2 mM EDTA, 2X PBS) and 50 μL streptavidin bead slurry and rotate end-to-end for 2 hours, centrifuge at 7000 rpm for 2 min. Wash 3 times with 1X Binding Buffer and 3 times with PBS. Add 30 μL 1X sample buffer to beads, heat samples at 95°C for 10 min. Run samples on an SDS-PAGE gel at 110V. After transferred, the membrane is immunoblotted with JNK antibody[1].
Cell Research JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100?U/mL) and streptomycin (100?g/mL), 2?mM L-gentamycin, and 50?μM 2-ME. These cells are stimulated with TNF (20?ng/mL), LPS (100?ng/mL), and IFN-γ (20?ng/mL), respectively. After 24 or 48?h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1?μM), NF-κB inhibitor (BAY 11-7082, 1?μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50?μM) are also added into the culture medium. After 48?h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].
Synonyms JNK inhibitor
Molecular Weight 493.56
Formula C28H27N7O2
CAS No. 1408064-71-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55 mg/mL (111.44 mM)

TargetMolReferences and Literature

1. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54. 2. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. 3. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC[J]. Endocrine-Related Cancer. 2020, 1(aop).

TargetMolCitations

1. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC. Endocrine-Related Cancer. 2020, 1(aop)

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Anti-Neurodegenerative Disease Compound Library Anti-Diabetic Compound Library MAPK Inhibitor Library Anti-Pancreatic Cancer Compound Library Inhibitor Library Anti-Ovarian Cancer Compound Library Bioactive Compounds Library Max HIF-1 Signaling Pathway Compound Library

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Keywords

JNK-IN-7 1408064-71-0 MAPK JNK Inhibitor JNK inhibitor inhibit JNK IN 7 JNKIN7 inhibitor

 

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