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Nocodazole

Catalog No. T2802 CAS 31430-18-9

Synonyms: R17934, Oncodazole

Nocodazole (Oncodazole) is a reversible inhibitor of microtubule polymerization and an inhibitor of Bcr-Abl. Nocodazole has antitumor activity, blocks the cell cycle and induces apoptosis.

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Nocodazole, CAS 31430-18-9

Pack Size	Availability	Price/USD
5 mg	In stock	$ 31.00
10 mg	In stock	$ 50.00
25 mg	In stock	$ 100.00
50 mg	In stock	$ 177.00
100 mg	In stock	$ 297.00
200 mg	In stock	$ 443.00
500 mg	In stock	$ 718.00
1 mL * 10 mM (in DMSO)	In stock	$ 34.00

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Purity: 99.91%

Purity: 99.34%

Purity: 98%

Biological Description

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Description	Nocodazole (Oncodazole) is a reversible inhibitor of microtubule polymerization and an inhibitor of Bcr-Abl. Nocodazole has antitumor activity, blocks the cell cycle and induces apoptosis.
Targets&IC50	Abl (T315I):0.64 μM, Abl:0.21 μM, Abl (E255K):0.53 μM
In vitro	METHODS: Erythrocytes were treated with Nocodazole (15-60 µg/mL) for 48 h. Phosphatidylserine was detected using Annexin-V-FITC. RESULTS: Nocodazole treatment increased the percentage of phosphatidylserine exposed to erythrocytes, reaching statistical significance at 30 µg/mL. [1] METHODS: hESCs cells were treated with Nocodazole (100 ng/mL) for 16 h and cell cycle profiles were analyzed using Flow Cytometry. RESULTS: Nocodazole treatment synchronized the cell cycle (>90% of cells in G2/M), while cells remained synchronized after release and moved evenly through the cell cycle for 24 h. At 2 h after removal of Nocodazole, the cells entered the G1 phase, with 70% of the cells in the G1 phase after 4 h, and 80% in the S phase after 12 h. The cells were treated with Nocodazole (100 ng/mL) for 16 h, and the cell cycle was analyzed by Flow Cytometry. [2]
In vivo	METHODS: To detect anti-tumor activity in vivo, Nocodazole (12 mg/kg three times a week) and dexamethasone (2 mg/kg twice a week) were injected intraperitoneally for fifteen days into immunodeficient mice bearing myeloma H929. RESULTS: Nocodazole in combination with dexamethasone significantly inhibited myeloma tumor growth and prolonged survival. [3] METHODS: To study the effects on intestinal mucositis, Nocodazole (5 mg/kg) and ketoconazole (50 mg/kg) were administered intraperitoneally three times a week for six weeks to nude mice harboring the human colorectal cancer tumor COLO 205. RESULTS: The antitumor effect of Nocodazole was significantly enhanced after six weeks of ketoconazole treatment. [4]
Cell Research	Nocodazole is dissolved in a final concentration of 0.05% DMSO. Proteins are loaded at 50 μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.

Synonyms	R17934, Oncodazole
Molecular Weight	301.32
Formula	C14H11N3O3S
CAS No.	31430-18-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 12.5 mg/mL (41.48 mM)

References and Literature

1. Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92. 2. Yiangou L, et al. Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics. Stem Cell Reports. 2019 Jan 8;12(1):165-179. 3. Feng R, et al. Targeting the microtubular network as a new antimyeloma strategy. Mol Cancer Ther. 2011 Oct;10(10):1886-96. 4. Wang YJ, et al. Ketoconazole potentiates the antitumor effects of nocodazole: In vivo therapy for human tumor xenografts in nude mice. Mol Carcinog. 2002 Aug;34(4):199-210. 5. Wang YJ, et al. Mol Carcinog. 2002, 34(4), 199-210. 6. Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92. 7. Zhang JP, et al. Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 2017 Feb 20;18(1):35. 8. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules[J]. Macromolecular bioscience. 2019: 1900105. 9. Reyes G X, Kolodziejczak A, Devakumar L J P S, et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair[J]. Current Biology. 10. Bai Y, Wang W, Li S, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex[J]. Molecular Cell. 2019.

Citations

1. Bai Y, Wang W, Li S, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. Molecular Cell. 2019 2. Moreira G G, Cantrelle F X, Quezada A, et al. Dynamic interactions and Ca2+-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding. Nature Communications. 2021, 12(1): 1-16 3. Reyes G X, Kolodziejczak A, Devakumar L J P S, et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Current Biology.. 2021 Mar 22;31(6):1268-1276.e6. doi: 10.1016/j.cub.2020.12.018. Epub 2021 Jan 7. 4. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules. Macromolecular bioscience. 2019: 1900105. 5. Cui L, Xu Q, Zhang P, et al.Biochemical reprogramming of active-targeting and redox responsive nanoparticles for enhancing selective internalization.Materials Letters.2023: 134379. 6. Wang J, Chen Y, Li S, et al.PP2A inhibition causes synthetic lethality in BRCA2-mutated prostate cancer models via reactivating spindle assembly checkpoint.The Journal of Clinical Investigation.2023

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Tyrosine Kinase Inhibitor Library Inhibitor Library Microtubule-Targeted Compound Library Anti-Neurodegenerative Disease Compound Library Human Metabolite Library Kinase Inhibitor Library Apoptosis Compound Library Bioactive Compounds Library Max Anti-Cancer Compound Library

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Keywords

Nocodazole 31430-18-9 Angiogenesis Apoptosis Autophagy Cytoskeletal Signaling DNA Damage/DNA Repair Tyrosine Kinase/Adaptors Microtubule Associated Bcr-Abl CRISPR/Cas9 Inhibitor R17934 Microtubule/Tubulin Oncodazole inhibit R 17934 R-17934 inhibitor

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