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Osalmid

Catalog No. T0353 CAS 526-18-1

Synonyms: Oxaphenamide, 4'-Hydroxysalicylanilide

Osalmid (Oxaphenamide) is a choleretic drug, inhibits ribonucleotide reductase activity by targeting ribonucleotide reductase small subunit M2 (RRM2).

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Osalmid, CAS 526-18-1

Pack Size	Availability	Price/USD
50 mg	In stock	$ 34.00
100 mg	In stock	$ 50.00
200 mg	In stock	$ 59.00
500 mg	In stock	$ 79.00
1 g	In stock	$ 97.00
1 mL * 10 mM (in DMSO)	In stock	$ 55.00

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Purity: 99.37%

Biological Description

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Description	Osalmid (Oxaphenamide) is a choleretic drug, inhibits ribonucleotide reductase activity by targeting ribonucleotide reductase small subunit M2 (RRM2).
Targets&IC50	RRM2:8.23 μM
In vitro	Osalmid has been identified as a potential compound targeting the ribonucleotide reductase small subunit M2 (RRM2), exhibiting a tenfold higher efficacy in inhibiting ribonucleotide reductase (RR) activity than hydroxyurea. It significantly suppresses both HBV DNA and cccDNA synthesis within HepG2.2.15 cells, following a time- and dose-dependent manner. The effective concentration (EC50) for inhibiting HBV DNA is noted as 11.1 μM in culture supernatant and 16.5 μM in cells, following an 8-day treatment with Osalmid, which demonstrates a concentration-dependent suppression of RR activity, marked by an IC50 of 8.23 μM. Moreover, Osalmid has demonstrated potent activity against a 3TC-resistant HBV strain, indicating its potential in treating drug-resistant HBV infections[1].
In vivo	Osalmid diminishes ribonucleotide reductase (RR) activity and hepatitis B virus (HBV) replication in HBV-transgenic mice, demonstrating synergistic effectiveness with lamivudine (3TC) while maintaining a low toxicity profile. Administered orally at a dosage of 400 mg/kg/day, osalmid progressively inhibits HBV DNA replication over time. A four-week treatment regimen results in a 40-45% decrease in HBV DNA replication levels in both the sera and liver tissues of mice, in comparison to untreated controls[1].
Cell Research	HepG2.2.15 cells are cultured in the presence of 200 μg/mL G418. Cell viability is determined using a Cell Counting Kit-8 in 96-well plates treated with Osalmid for designated times. For long term assays, the culture supernatants are replaced with fresh media containing Osalmid every two days. The control wells contained equivalent amounts of DMSO. The CC50 is calculated as the concentration of a compound that reduced the cell viability to 50% compared to the control[1].

Synonyms	Oxaphenamide, 4'-Hydroxysalicylanilide
Molecular Weight	229.23
Formula	C13H11NO3
CAS No.	526-18-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (218.12 mM)

Ethanol: 41 mg/mL(178.9 mM)

References and Literature

1. Liu X, et al. Inhibition of hepatitis B virus replication by targeting ribonucleotide reductase M2 protein. Biochem Pharmacol. 2016 Mar 1;103:118-28. 2. Ivanova L, Rausalu K, Žusinaite E, et al. 1, 3-Thiazolbenzamide Derivatives as Chikungunya Virus nsP2 Protease Inhibitors[J]. ACS Omega. 2021

Citations

1. Ivanova L, Rausalu K, Žusinaite E, et al. 1,3-Thiazolbenzamide Derivatives as Chikungunya Virus nsP2 Protease Inhibitors. ACS Omega. 2021, 6(8): 5786-5794 2. Wang R, Xie S, Zhu S, et al. Targeting matrix metallopeptidase 2 by hydroxyurea selectively kills acute myeloid mixed-lineage leukemia. Cell Death Discovery. 2022, 8(1): 1-10 3. Ivanova L, Rausalu K, Žusinaite E, et al. 1, 3-Thiazolbenzamide Derivatives as Chikungunya Virus nsP2 Protease Inhibitors. ACS Omega. 2021 Feb 17;6(8):5786-5794. doi: 10.1021/acsomega.0c06191. eCollection 2021 Mar 2.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Inhibitor Library Toxic Compound Library ReFRAME Related Library Anti-COVID-19 Compound Library Hematonosis Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Osalmid 526-18-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA/RNA Synthesis HBV Oxaphenamide Hepatitis B virus 4'-Hydroxysalicylanilide inhibit Inhibitor inhibitor

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