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Penciclovir

Catalog No. T1643   CAS 39809-25-1
Synonyms: BRL 39123, VSA 671

Penciclovir (BRL 39123) is a Herpesvirus Nucleoside Analog DNA Polymerase Inhibitor. In HSV infected cells, penciclovir is phosphorylated by viral thymidine kinase and subsequently converted by cellular kinases into the active metabolite, penciclovir triphosphate, which competitively inhibits viral HSV polymerase by blocking deoxyguanosine triphosphate substrate binding. As a result, herpes viral DNA synthesis and replication are selectively inhibited.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Penciclovir Chemical Structure
Penciclovir, CAS 39809-25-1
Pack Size Availability Price/USD Quantity
100 mg In stock $ 30.00
200 mg In stock $ 43.00
500 mg In stock $ 68.00
1 mL * 10 mM (in DMSO) In stock $ 29.00
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Purity: 99.1%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Penciclovir (BRL 39123) is a Herpesvirus Nucleoside Analog DNA Polymerase Inhibitor. In HSV infected cells, penciclovir is phosphorylated by viral thymidine kinase and subsequently converted by cellular kinases into the active metabolite, penciclovir triphosphate, which competitively inhibits viral HSV polymerase by blocking deoxyguanosine triphosphate substrate binding. As a result, herpes viral DNA synthesis and replication are selectively inhibited.
Targets&IC50 HSV types 1:0.04-1.8 μg/mL, HSV types 2:0.06-4.4 μg/mL
Cell Research Penciclovir is dissolved with DMSO and diluted with appropriate media[2]. Human breast cancer cell lines MCF-7 and MDA-MB-435, glioblastoma U87 mg, and embryonic kidney cells 293T are cultured at 37°C in a humidified atmosphere containing 5% CO2 in Iscove's modified Dulbecco medium or Leibovitz's L-medium and 5% fetal bovine serum (FBS). The assays are performed with slight modifications. In brief, cells are seeded into 24-well plates (5×104 cells/well) and infected 48 h later with 103 particles per cell of unmodified virus (Adtk), PEGylated virus (PEG-Adtk), or RGD-PEG-modified virus (RGD-PEG-Adtk) in triplicates in culture medium with 2% FBS and incubated for 4 h at 37°C. The incubation medium is then replaced by normal medium and cells are further incubated for 48 h. Cells are harvested and lysed with 500 μL of TK lysis buffer that contained 0.5% Nonidet P-40 (NP-40), 20 mM N-(2-hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) (HEPES) (pH 7.6), 2 mM Mg(OAc)2, 1 mM dithiothreitol, and 50 μM thymidine. The supernatant is collected after centrifugation. The samples are kept at -80°C until use. The modified and unmodified adenovirus protein concentrations are determined by the Micro BCA assay. One microgram of cell extract is incubated with HSV1-tk substrate 8-3H-Penciclovir (8-3 H-PCV). The phosphorylated tracer is separated from unphosphorylated 8-3 H-PCV with DE-81 filters. TK activity is expressed as the percentage of conversion of substrate per minute per microgram protein[2].
Synonyms BRL 39123, VSA 671
Molecular Weight 253.26
Formula C10H15N5O3
CAS No. 39809-25-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 1 mg/mL (3.94 mM)

DMSO: 32 mg/mL (126.4 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Earnshaw DL, et al. Antimicrob Agents Chemother, 1992, 36(12), 2747-2757. 2. Amjad M, et al. Microbiol Immunol, 2001, 45(3), 233-240. 3. Thust R, et al. Cancer Gene Ther, 2000, 7(1), 107-117. 4. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination[J]. Antiviral Research. 2020: 104931

TargetMolCitations

1. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination. Antiviral Research. 2020: 104931

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compounds Library Max Anti-Infection Compound Library ReFRAME Related Library Toxic Compound Library FDA-Approved & Pharmacopeia Drug Library Anti-Viral Compound Library NO PAINS Compound Library Approved Drug Library Inhibitor Library Clinical Compound Library

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Keywords

Penciclovir 39809-25-1 Microbiology/Virology Proteases/Proteasome Antifection HCV Protease HSV VSA671 BRL-39123 BRL 39123 mortality inhibit no-toxic Inhibitor VSA 671 anti-herpesvirus VSA-671 BRL39123 Herpes simplex virus inhibitor

 

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