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Torin 2

Catalog No. T6100   CAS 1223001-51-1
Synonyms: DNA-dependent protein kinase, mTOR, Autophagy, Torin-2, inhibit, Mammalian target of Rapamycin, DNA-PK, Torin 2, Torin2, Inhibitor, Apoptosis

Torin 2(IC50=0.25 nM), a specific and effective mTOR inhibitor, is the 800-fold greater specific activity for mTOR than PI3K and improves pharmacokinetic properties. The EC50 of Torin 2 for ATM/ATR/DNA-PK inhibition is 28 nM/35 nM/118 nM, respectively.

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Torin 2, CAS 1223001-51-1
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Purity: 98%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Torin 2(IC50=0.25 nM), a specific and effective mTOR inhibitor, is the 800-fold greater specific activity for mTOR than PI3K and improves pharmacokinetic properties. The EC50 of Torin 2 for ATM/ATR/DNA-PK inhibition is 28 nM/35 nM/118 nM, respectively.
Targets&IC50 ATM:28 nM(EC50), DNA-PK:118 nM(EC50), ATR:35 nM(EC50), mTOR:0.25 nM
Kinase Assay mTOR and PI3K Cellular Assays: Cellular IC50 values for mTOR are determined using p53?/? MEFs. Cells are treated with vehicle or increasing concentrations of Torin 2 for 1 h and then lyse. Phosphorylation of S6K1 Thr-389 is monitored by immunoblotting using a phospho-specific antibody. Meanwhile, cellular IC50 values for PI3Ka are determined based on phosphorylation of Akt Thr-308 in p53?/?/mLST8?/? MEFs or human PC3 cells expressing the S473D mutant of Akt1.
Cell Research For viability, MZ-CRC-1 and TT cells are seeded in quadruplicate in 96-well plates (1.0×104 cells per well) in culture media with 2.5% and 4% FBS, respectively. After 24 hours, cells are treated with Torin 2. At the indicated time point, cells are incubated for 3 hours with 10 μL of CellTiter96 AQueous One solution in 100 μL of culture media and absorbance is measured at 490 nm.(Only for Reference)
Synonyms DNA-dependent protein kinase, mTOR, Autophagy, Torin-2, inhibit, Mammalian target of Rapamycin, DNA-PK, Torin 2, Torin2, Inhibitor, Apoptosis
Molecular Weight 432.406
Formula C24H15F3N4O
CAS No. 1223001-51-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 8.7 mg/mL (20 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Liu Q, et al. J Med Chem, 2011, 54(5), 1473-1480. 2. Settembre C, et al. EMBO J, 2012, 31(5), 1095-1108. 3. Tamburrino A, et al. Clin Cancer Res, 2012, 18(13), 3532-3540. 4. Berry T, et al. Cancer Cell, 2012, 22(1), 117-130. 5. Liu Q, et al. Cancer Res, 2013, 73(8), 2574-2586. 6. Wang X, et al. mTORC signaling in hematopoiesis. Int J Hematol. 2016 May;103(5):510-8.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Compound Library Autophagy Compound Library Anti-Lung Cancer Compound Library HIF-1 Signaling Pathway Compound Library Cancer Cell Differentiation Compound Library Glycometabolism Compound Library Anti-Cancer Metabolism Compound Library Anti-Obesity Compound Library Glutamine Metabolism Compound Library Anti-Pancreatic Cancer Compound Library

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