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Amonafide

🥰Excellent
Catalog No. T6336Cas No. 69408-81-7
Alias Quinamed, NSC308847, Nafidimide, AS1413

Amonafide (NSC-308847,AS1413)(AS1413) produces protein-associated DNA-strand breaks through a topoisomerase II-mediated reaction, but does not produce topoisomerase I-mediated DNA cleavage.

Amonafide

Amonafide

🥰Excellent
Purity: 99.39%
Catalog No. T6336Alias Quinamed, NSC308847, Nafidimide, AS1413Cas No. 69408-81-7
Amonafide (NSC-308847,AS1413)(AS1413) produces protein-associated DNA-strand breaks through a topoisomerase II-mediated reaction, but does not produce topoisomerase I-mediated DNA cleavage.
Pack SizePriceAvailabilityQuantity
1 mg31 €In Stock
2 mg44 €In Stock
5 mg69 €In Stock
10 mg84 €In Stock
25 mg157 €In Stock
50 mg281 €In Stock
100 mg466 €In Stock
1 mL x 10 mM (in DMSO)79 €In Stock
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Purity:99.39%
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Product Introduction

Bioactivity
Description
Amonafide (NSC-308847,AS1413)(AS1413) produces protein-associated DNA-strand breaks through a topoisomerase II-mediated reaction, but does not produce topoisomerase I-mediated DNA cleavage.
In vitro
Through a topoisomerase II-mediated reaction, Amonafide treatment produces DNA single-strand breaks (SSB), double-strand breaks (DSB), and DNA-protein cross-links in human myeloid leukemia cells. Amonafide treatment inhibits conlony formation of the leukemic cell lines and the normal human bone marrow GM-CFC in a dose-dependent manner. Amonafide does not produce topoisomerase I-mediated DNA cleavage even at 100 μM. The m-AMSA-resistant line is less than 2-fold resistant to Amonafide [1] Amonafide interferes with the DNA breakage-reunion activity of mammalian DNA topoisomerase II resulting in DNA cleavage stimulation. [2] Compared with those of other antitumor drugs, Amonafide-stimulated cleavage intensity patterns are markedly different. Amonafide highly prefers a cytosine, and excludes guanines and thymines instead, at position -1, with lower preference for an adenine at position +1. [3] Topoisomerase II-mediated DNA cleavage induced by Amonafide is affected only slightly (less than 3-fold) by 1 mM ATP, suggeting that Amonafide is an ATP-insensitive topoisomerase II inhibitor in contrast to doxorubicin, etoposide, and mitoxantrone. [4] Amonafide significantly inhibits the growth of HT-29, HeLa, and PC3 cells with IC50 of 4.67 μM, 2.73 μM, and 6.38 μM, respectively. [5] Amonafide is unaffected by P-glycoprotein-mediated efflux, unlike those of the classical topoisomerase II inhibitors (daunorubicin, doxorubicin, idarubicin, etoposide, and mitoxantrone). [6]
Cell Research
All cell lines are in the logarithmic phase of growth when the assay of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is carried out. Cells are harvested and seeded into 96-well tissue culture plates at a density of 2.5 × 103 cells/well in 150 μL aliquots of medium. The concentrations tested are serial dilutions of a stock solution (10 μM in DMSO) with phosphate-buffered saline (PBS) and are added 24 hours later. The assay is ended after 72 hours of Amonafide exposure and PBS is used as a negative control. After 72 hours treatment, cells are washed twice with PBS, and then 50 μL/well of MTT reagent (1 mg/mL in PBS) together with 150 μL/well of prewarmed medium are added. The plates are returned to the incubator for 4 hours. Subsequently, DMSO is added as solvent. Absorbance is determined at 570 nm with a Microplate reader. All experiments are performed at least three times, and the average of the percentage absorbance is plotted against concentration. Then, the concentration of Amonafide required to inhibit 50% of cell growth (IC50) is calculated for Amonafide.(Only for Reference)
AliasQuinamed, NSC308847, Nafidimide, AS1413
Chemical Properties
Molecular Weight283.33
FormulaC16H17N3O2
Cas No.69408-81-7
SmilesCN(C)CCN1C(=O)c2cccc3cc(N)cc(C1=O)c23
Relative Density.1.306 g/cm3 (Predicted)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 53 mg/mL (187.1 mM)
Ethanol: 4 mg/mL (14.11 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM3.5295 mL17.6473 mL35.2945 mL176.4727 mL
5 mM0.7059 mL3.5295 mL7.0589 mL35.2945 mL
10 mM0.3529 mL1.7647 mL3.5295 mL17.6473 mL
DMSO
1mg5mg10mg50mg
20 mM0.1765 mL0.8824 mL1.7647 mL8.8236 mL
50 mM0.0706 mL0.3529 mL0.7059 mL3.5295 mL
100 mM0.0353 mL0.1765 mL0.3529 mL1.7647 mL

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