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Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $86 | In Stock | |
2 mg | $127 | In Stock | |
5 mg | $198 | In Stock | |
10 mg | $373 | In Stock | |
25 mg | $628 | In Stock | |
50 mg | $893 | In Stock | |
100 mg | $1,220 | In Stock | |
500 mg | $2,450 | In Stock |
Description | MPI-0479605 is an ATP competitive and selective inhibitor. |
Targets&IC50 | Mps1:1.8 nM |
Kinase Assay | In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel. |
Cell Research | Cells are treated for 3 or 7 days with various concentrations of MPI-0479605 and the GI50 is determined. Cell viability is measured with CellTiter-Glo. (Only for Reference) |
Molecular Weight | 407.51 |
Formula | C22H29N7O |
Cas No. | 1246529-32-7 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||
Solubility Information | DMSO: 1 mg/ml, Sonication is recommended. ![]() Ethanol: 2 mg/mL (4.9 mM), Heating is recommended. | ||||||||||
Solution Preparation Table | |||||||||||
Ethanol
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