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4EGI-1

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Purity:99.12%

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COA HPLC HNMR

4EGI-1

Catalog No. T2665Cas No. 315706-13-9

4EGI-1, a competitive eIF4E/eIF4 g interaction inhibitor, binds to eIF4E(KD=25 μM).

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Pack Size	Price	Availability
1 mg	$37	In Stock
2 mg	$50	In Stock
5 mg	$90	In Stock
10 mg	$117	In Stock
25 mg	$231	In Stock
50 mg	$369	In Stock
1 mL x 10 mM (in DMSO)	$98	In Stock

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Product Introduction

Bioactivity

Description	4EGI-1, a competitive eIF4E/eIF4 g interaction inhibitor, binds to eIF4E(KD=25 μM).
Targets&IC50	eIF4E-eIF4G:25 μM(Kd)
In vivo	In Jurkat cells, 4EGI-1 promotes apoptosis. It effectively inhibits cell growth in A549 lung cancer cells (IC50=6 μM). In human lung cancer cells, 4EGI-1 enhances TRAIL-induced apoptosis by inducing DR5 and downregulating c-FLIP, thereby inhibiting cap-dependent protein translation.
Cell Research	Cell viability is measured by treatment of Jurkat cells with compound for 24 h and by determination of intracellular ATP using the CellTiterGlo assay. For measurement of apoptotic DNA fragmentation, cells are treated for 24 h with 60 μM EGI-1 or 6.65 μM camptothecin in the presence or absence of 100 mM zVAD-FMK, a broad-spectrum caspase inhibitor. After fixation and staining with PI, cellular DNA content is determined by FACS analysis in a FACS Calibur machine. Nuclear morphology after 24 h EG1-1 treatment is visualized by staining of cells with Hoechst dye and ?uorescence microscopy. For the A549 lung cancer cells, cell growth in the presence of 4EGI-1 is determined using the SRB staining method.(Only for Reference)
Alias	4EGI 1

Chemical Properties

Molecular Weight	451.28
Formula	C18H12Cl2N4O4S
Cas No.	315706-13-9

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 45.1 mg/mL (100 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2159 mL	11.0796 mL	22.1592 mL	110.7960 mL
5 mM	0.4432 mL	2.2159 mL	4.4318 mL	22.1592 mL
10 mM	0.2216 mL	1.1080 mL	2.2159 mL	11.0796 mL
20 mM	0.1108 mL	0.5540 mL	1.1080 mL	5.5398 mL
50 mM	0.0443 mL	0.2216 mL	0.4432 mL	2.2159 mL
100 mM	0.0222 mL	0.1108 mL	0.2216 mL	1.1080 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc