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Calpeptin

Catalog No. T6432 CAS 117591-20-5

Calpeptin is a potent, cell-permeable calpain inhibitor.

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Calpeptin, CAS 117591-20-5

Pack Size	Availability	Price/USD
5 mg	In stock	$ 52.00
10 mg	In stock	$ 76.00
25 mg	In stock	$ 162.00
50 mg	In stock	$ 228.00
100 mg	In stock	$ 372.00
1 mL * 10 mM (in DMSO)	In stock	$ 57.00

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Purity: 98.33%

Purity: 97.52%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Calpeptin is a potent, cell-permeable calpain inhibitor.
Targets&IC50	Calpain I (porcine erythrocytes):52 nM(ID50), Papainb:138 nM(ID50), Calpain II (porcine kidney):34 nM(ID50), Calpain I (human platelets):40 nM(ID50)
In vitro	Calpeptin inhibits 20K phosphorylation in a dose-related manner in platelets stimulated by thrombin, ionomycin or collagen. [1] In differentiating PC12 cells, Calpeptin promotes neurite elongation via inhibition of Calpain activity. [2] In rat retinal ganglion cells, Calpeptin attenuates apoptosis, maintains normal whole-cell membrane potential, and thus provides functional neuroprotection. [3]
In vivo	In a feline right ventricular (RV) PO (RVPO) model, calpeptin (0.6 mg/kg, i.v.) blocks the activation of calpain and caspase-3, cleavage of their substrates, and cardiomyocyte programmed cell death. [4] In a rat focal cerebral ischemia–reperfusion injury model, Calpeptin reduces the neuronal apoptosis in hippocampal CA1 sector via inhibition of the expression of Caspase-3. [5]

Molecular Weight	362.46
Formula	C20H30N2O4
CAS No.	117591-20-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 67 mg/mL (184.8 mM)

DMSO: 67 mg/mL (184.8 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Tsujinaka T, et al. Biochem Biophys Res Commun. 1988, 153(3), 1201-1208. 2. Pintér M, et al. Neurosci Lett. 1994 Mar 28;170(1):91-3. 3. Das A, et al. Brain Res. 2006, 1084(1), 146-157. 4. Mani SK, et al. Am J Physiol Heart Circ Physiol. 2008, 295(1), H314-326. 5. Peng S, et al. Mol Biol Rep. 2011, 38(2), 905-912.

Citations

1. Chen Y, Chen L, Wu X, et al.Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice.Nature Communications.2023, 14(1): 1521. 2. Tian T, Xie X, Yi W, et al.FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation.Cell Reports.2023, 42(11). 3. Zhang J, Jiang T, Zhang Y, et al.Phillygenin prevents osteoclast differentiation and bone loss by targeting RhoA.Phytotherapy Research.2024

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Apoptosis Compound Library HIF-1 Signaling Pathway Compound Library Preclinical Compound Library Protease Inhibitor Library Bioactive Compound Library Covalent Inhibitor Library NO PAINS Compound Library Ubiquitination Compound Library Bioactive Compounds Library Max

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Calpeptin 117591-20-5 Apoptosis Proteases/Proteasome Ubiquitination Proteasome Cysteine Protease Cathepsin inhibit Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.