Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMH-1 is a potent and selective BMP inhibitor.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 53.00 | |
10 mg | In stock | $ 84.00 | |
25 mg | In stock | $ 163.00 | |
50 mg | In stock | $ 291.00 | |
100 mg | In stock | $ 455.00 | |
500 mg | In stock | $ 987.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 63.00 |
Description | DMH-1 is a potent and selective BMP inhibitor. |
Targets&IC50 | ALK2:107.9 nM |
Kinase Assay | Kinase assay: All kinase assays are conducted by Reaction Biology Corp. In brief, compounds are tested at 10 concentrations by 3-fold serial dilutions starting at 30 μM, using nonspecific kinase inhibitor staurosporine as control. In vitro kinase reactions are carried out in the presence of 10 μM (33P)γATP. Five kinases tested are the human BMP type-I receptor activin receptor-like kinase 2 (ALK-2/ACVR1), the human TGFβ type-I receptor activin receptor-like kinase 5 (Alk5/TGFβR1), the human VEGF type-II receptor (KDR/Flk-1/VEGFR2), the human AMP-activated protein kinase (AMPK/A1/B1/G1) and the human platelet-derived growth factor receptor-β (PDGFRβ). |
Cell Research | About 10,000 A549 cells per well are seeded in 96-well plates and incubated for overnight. The culture medium is then changed to fresh medium containing DMSO or DMH1 at various concentrations. The cells are then incubated for 48 hours and 96 hours before treatment termination by replacing the medium with 100 μL of 10% trichloroacetic acid in 1× PBS, followed by incubation at 4°C for at least 1 hour. Subsequently, the plates are washed with water and air dried. The plates are stained with 50 μL 0.4% sulphorhodamine assay in 1% acetic acid for 30 minutes at room temperature. Unbound dye is washed off with 1% acetic acid. After air drying and solubilization of the protein-bound dye in 10 mM Tris solution, absorbance is read in a microplate reader at 565 nm.(Only for Reference) |
Synonyms | DMH1 |
Molecular Weight | 380.44 |
Formula | C24H20N4O |
CAS No. | 1206711-16-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 12.5 mg/mL (32.86 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
DMH-1 1206711-16-1 Angiogenesis Autophagy Stem Cells Tyrosine Kinase/Adaptors TGF-beta/Smad ALK Inhibitor Transforming growth factor beta receptors DMH 1 inhibit TGF-β Receptor DMH1 inhibitor