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Gimeracil

Catalog No. T0987 CAS 103766-25-2

Synonyms: Gimestat

Gimeracil (Gimestat) is a competitive, reversible inhibitor of dihydropyrimidine dehydrogenase.

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Gimeracil, CAS 103766-25-2

Pack Size	Availability	Price/USD
25 mg	In stock	$ 51.00
50 mg	In stock	$ 68.00
100 mg	In stock	$ 90.00
200 mg	In stock	$ 110.00
500 mg	In stock	$ 131.00
1 mL * 10 mM (in DMSO)	In stock	$ 54.00

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Purity: 99.86%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Gimeracil (Gimestat) is a competitive, reversible inhibitor of dihydropyrimidine dehydrogenase.

Kinase Assay

Microdilution method: The culture media used are RPMI 1640 with glutamine, without bicarbonate and phenol red, buffered with morpholinopropanesulfonic acid (MOPS) (0.165 M, pH 7.0). Two-fold serial dilutions of Flucytosine (0.06-64 μg/mL) are prepared and dispensed in 50 uL aliquot, in flat-bottom 96-well assay plates which are kept frozen at -70 °C in sealed plastic bags until used. The inoculum is prepared spectrophotometrically and standardized to a concentration of 1.0-5.0 × 103 cfu per mL. A 50 μL volume of this suspension is used to inoculate each well containing 50 μL of the double concentration of Flucytosine to be tested. Once inoculated, each well therefore contains 100 μL of broth favoured over 200 μL to facilitate the agitation of the plates prior to spectrophotometric reading. After an incubation period of 24 and 48 hours at 35 °C, the plates are agitated for 3 minutes at 900 r.p.m. with a shaker and the optical density of the growth in each well is determined with the use of an automatic plate reader set at 495 nm. The inhibitory concentration of IC50 is computed mathematically.

Synonyms	Gimestat
Molecular Weight	145.54
Formula	C5H4ClNO2
CAS No.	103766-25-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 28 mg/mL (192.4 mM)

References and Literature

1. Prescrire Int. 2013 May;22(138):122.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Drug Library Anti-Cancer Active Compound Library Anti-Cancer Approved Drug Library EMA Approved Drug Library Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library Inhibitor Library Human Metabolite Library High Solubility Micro Fragment Library Clinical Compound Library

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Gimeracil 103766-25-2 Autophagy Lu-99 inhibit Inhibitor Gimestat LC-11 repair DNA PAN-4 KB/C3 radiotherapy inhibitor

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