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Pack Size | Price | Availability | Quantity |
---|---|---|---|
5 mg | $32 | In Stock | |
10 mg | $52 | In Stock | |
25 mg | $85 | In Stock | |
50 mg | $126 | In Stock | |
100 mg | $198 | In Stock | |
200 mg | $237 | In Stock | |
500 mg | $398 | In Stock | |
1 mL x 10 mM (in DMSO) | $41 | In Stock |
Description | Ataluren (PTC124) is a novel, orally administered drug that targets nonsense mutations. Ataluren is approved for use by the European Medicines Agency to treat Duchenne Muscular Dystrophy in patients aged 5 years and older who are able to walk. |
Cell Research | PTC124 (Ataluren) is prepared in DMSO and stored, and then diluted with appropriate medium (DMSO 1%) before use[2]. Duplicate samples of HEK293 cells harbouring LUC-190 (UGA) are incubated in the presence of 5?μM PTC124 (treated) or 1% DMSO (untreated) for 20?h. The cells are collected, washed twice in phosphate buffered saline (PBS), resuspended in sample buffer (Bio-Rad) and shipped on dry ice to Kendrick Laboratories for two-dimensional electrophoretic analysis Isoelectric focusing (pH?3.5-10) is carried out in glass tubes for 20,000 V-hours. One?μg of a tropomyosin internal standard is added to each sample. Second dimension SDS slab gel electrophoresis is carried out for approximately 6?h at 25?mA per gel. After electrophoresis, gels are transferred to PVDF paper. Computerized analysis of spot mobility used Phoretix software[2]. |
Alias | PTC124 |
Molecular Weight | 284.24 |
Formula | C15H9FN2O3 |
Cas No. | 775304-57-9 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | H2O: < 1 mg/mL (insoluble or slightly soluble) Ethanol: < 1 mg/mL (insoluble or slightly soluble) DMSO: 40 mg/mL (140.73 mM), Sonication is recommended. ![]() | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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