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Epothilone A

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Purity:99.48%

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COA HPLC HNMR

Epothilone A

Catalog No. T6490Cas No. 152044-53-6

Epothilone A (Epo A) is a microtubule-stabilizing agent with EC0.01 of 2 μM.

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Pack Size	Price	Availability
1 mg	$31	In Stock
2 mg	$44	In Stock
5 mg	$72	In Stock
10 mg	$118	In Stock
25 mg	$239	In Stock
50 mg	$363	In Stock
100 mg	$547	In Stock
200 mg	$779	In Stock
1 mL x 10 mM (in DMSO)	$79	In Stock

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Product Introduction

Bioactivity

Description	Epothilone A (Epo A) is a microtubule-stabilizing agent with EC0.01 of 2 μM.
In vivo	Epothilone A induces microtubule polymerization, causing cell cycle arrest at the G2-M phase, and exhibits cytotoxicity, as well as induces apoptosis. It effectively inhibits the proliferation of HCT116 cells with an IC50 of 4.4 nM. Epothilone A possesses cytotoxicity against KB3-1, KBV-1, Hela, and Hs578T cells with IC50 values ranging from 13 nM to 160 nM. Additionally, Epothilone A competitively binds to microtubules against paclitaxel, with an IC50 of 2.3 μM.
Kinase Assay	Tubulin polymerization assay: Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone A is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope.
Cell Research	Cell lines: KB3-1,KBV-1,Hela,and Hs578T cells. Concentrations: 0–2 μM. Method: cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips.After 24 hours,cells are treated with Epothilone A and are scored at regular intervals.For the cytotoxicity analysis,cells are counted and scored as trypan blue positive or negative.
Alias	epothile, Epo A

Chemical Properties

Molecular Weight	493.66
Formula	C26H39NO6S
Cas No.	152044-53-6

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 92 mg/mL (186.4 mM)

DMSO: 92 mg/mL (186.4 mM)

Solution Preparation Table

Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.0257 mL	10.1284 mL	20.2569 mL	101.2843 mL
5 mM	0.4051 mL	2.0257 mL	4.0514 mL	20.2569 mL
10 mM	0.2026 mL	1.0128 mL	2.0257 mL	10.1284 mL
20 mM	0.1013 mL	0.5064 mL	1.0128 mL	5.0642 mL
50 mM	0.0405 mL	0.2026 mL	0.4051 mL	2.0257 mL
100 mM	0.0203 mL	0.1013 mL	0.2026 mL	1.0128 mL

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In Vivo Formulation Calculator (Clear solution)

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DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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