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KN-62

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Purity:100%

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COA LCMS HNMR

KN-62

Catalog No. T2694Cas No. 127191-97-3

KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.

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Pack Size	Price	Availability
1 mg	$30	In Stock
2 mg	$44	In Stock
5 mg	$68	In Stock
10 mg	$113	In Stock
25 mg	$229	In Stock
50 mg	$448	In Stock
100 mg	$658	In Stock
1 mL x 10 mM (in DMSO)	$113	In Stock

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Product Introduction

Bioactivity

Description	KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.
In vitro	KN-62 administration in adult rats reduces the expression levels of brain-derived neurotrophic factor (BDNF) mRNA induced by epilepsy in the brain.
In vivo	KN-62 inhibits the proliferation of K562 cells and blocks cell cycle progression. When administered at 10 μM to rat pancreatic islet cells, KN-62 inhibits insulin secretion stimulated by carbachol and potassium. Furthermore, KN-62 suppresses the phosphorylation of Ca2+/CaM kinase induced by A23187 in PC12 D cells.
Kinase Assay	Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid.
Cell Research	For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference)

Chemical Properties

Molecular Weight	721.84
Formula	C38H35N5O6S2
Cas No.	127191-97-3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 72.2 mg/mL (100 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3853 mL	6.9267 mL	13.8535 mL	69.2674 mL
5 mM	0.2771 mL	1.3853 mL	2.7707 mL	13.8535 mL
10 mM	0.1385 mL	0.6927 mL	1.3853 mL	6.9267 mL
20 mM	0.0693 mL	0.3463 mL	0.6927 mL	3.4634 mL
50 mM	0.0277 mL	0.1385 mL	0.2771 mL	1.3853 mL
100 mM	0.0139 mL	0.0693 mL	0.1385 mL	0.6927 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

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Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

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