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JNK-IN-7

JNK-IN-7
JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
Catalog No. T3598Cas No. 1408064-71-0
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Purity:99.58%
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JNK-IN-7

Purity: 99.58%
Catalog No. T3598Alias JNK inhibitorCas No. 1408064-71-0

JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
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Pack SizePriceAvailabilityQuantity
1 mg$89In Stock
2 mg$161In Stock
5 mg$238In Stock
10 mg$306In Stock
25 mg$550In Stock
50 mg$792In Stock
100 mg$1,080In Stock
1 mL x 10 mM (in DMSO)$258In Stock
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Product Introduction

Bioactivity
Description
JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
Targets&IC50
JNK2:1.99nM, JNK3:0.75 nM, JNK1:1.54 nM
In vitro
JNK-IN-7 is a relatively selective JNK inhibitor in cells, binding to JNK1, 2, 3, IRAK1 (IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3, and PIP4K2C[1]. In HCT116 cells, TNF stimulation for 24 and 48 hours significantly decreases divalent metal-ion transporter 1 (DMT1) expression, a process that JNK-IN-7 can notably counteract[2].
Kinase Assay
A375 cells are pre-treated with 1 μM JNK-IN-7 for the indicated amounts of time. Remove the medium and wash 3 times with PBS. Resuspend the cell pellet with 1 mL Lysis Buffer (1% NP-40, 1% CHAPS, 25 mM Tris, 150 mM NaCl, Phosphatase Inhibitor Cocktail). Rotate end-to-end for 30 min at 4°C. Lysates are cleared by centrifugation at 14000 rpm for 15 min in the Eppendorf. The cleared lysates gel filtered into Kinase Buffer (0.1% NP-40, 20 mM HEPES, 150 mM NaCl, Phosphatase Inhibitor Cocktail, Protease Inhibitor Cocktail) using Bio-Rad 10DG colums. The total protein concentration of the gel-filtered lysate should be around 5-15 mg/mL. Cell lysate is labeled with the probe at 5 μM for 1 hour. Samples are reduced with DTT, and cysteines are blocked with iodoacetamide and gel filtered to remove excess reagents and exchange the buffer. Add 1 volume of 2X Binding Buffer (2% Triton-100, 1% NP-40, 2 mM EDTA, 2X PBS) and 50 μL streptavidin bead slurry and rotate end-to-end for 2 hours, centrifuge at 7000 rpm for 2 min. Wash 3 times with 1X Binding Buffer and 3 times with PBS. Add 30 μL 1X sample buffer to beads, heat samples at 95°C for 10 min. Run samples on an SDS-PAGE gel at 110V. After transferred, the membrane is immunoblotted with JNK antibody[1].
Cell Research
JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100?U/mL) and streptomycin (100?g/mL), 2?mM L-gentamycin, and 50?μM 2-ME. These cells are stimulated with TNF (20?ng/mL), LPS (100?ng/mL), and IFN-γ (20?ng/mL), respectively. After 24 or 48?h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1?μM), NF-κB inhibitor (BAY 11-7082, 1?μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50?μM) are also added into the culture medium. After 48?h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].
AliasJNK inhibitor
Chemical Properties
Molecular Weight493.56
FormulaC28H27N7O2
Cas No.1408064-71-0
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 55 mg/mL (111.44 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.0261 mL10.1305 mL20.2610 mL101.3048 mL
5 mM0.4052 mL2.0261 mL4.0522 mL20.2610 mL
10 mM0.2026 mL1.0130 mL2.0261 mL10.1305 mL
20 mM0.1013 mL0.5065 mL1.0130 mL5.0652 mL
50 mM0.0405 mL0.2026 mL0.4052 mL2.0261 mL
100 mM0.0203 mL0.1013 mL0.2026 mL1.0130 mL

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