Home Tools
Log in
Cart

MI-136

Catalog No. T6889   CAS 1628316-74-4

MI-136 inhibits expression of androgen receptor (AR) target genes that DHT induced.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
MI-136 Chemical Structure
MI-136, CAS 1628316-74-4
Pack Size Availability Price/USD Quantity
1 mg In stock $ 39.00
2 mg In stock $ 57.00
5 mg In stock $ 89.00
10 mg In stock $ 150.00
25 mg In stock $ 293.00
50 mg In stock $ 531.00
100 mg In stock $ 768.00
1 mL * 10 mM (in DMSO) In stock $ 98.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 98.63%
Purity: 97.08%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description MI-136 inhibits expression of androgen receptor (AR) target genes that DHT induced.
In vitro MI-136, a variant of a previously described inhibitor that can specifically inhibit the menin-MLL interaction. AR positive cell lines such as VCaP, LNCaP and 22RV1 are sensitive to MI-136. Treatment with MI-136 also inhibits the expression of genes that are bound to ASH2L after AR stimulation. Treatment with MI-136 induces apoptosis of VCaP cells as evidenced by PARP (cPARP) cleavage and blocks DHT-induced cell proliferation in AR-dependent cell lines (LNCaP and VCaP). The effect of MI-136 on cell proliferation is similar to MDV-3100, a second-generation FDA-approved anti-androgen for patients with refractory prostate cancer[1].
In vivo Treatment of VCaP tumor-bearing mice with MI-136 (40 mg/kg) leads to a modest but significant reduction in tumor volume with no effect on mouse body weight[1].
Kinase Assay HSP90 binding, ATPase, and selectivity profiling assays: The potency of HSP90 inhibitors for HSP90α, HSP90β, and Grp94 is determined by AlphaScreen competition binding assays, and activity against TRAP-1 is assessed by an ATPase assay.
Cell Research To assess the effect of MI-136 on AR signaling, VCaP cells are treated with DMSO or 5 μM MI-136 for 48 hours. Cells are serum starved by replacing the media with DMEM containing 5% charcoal-striped serum and MI-136 for 48 hrs. Cells are then stimulated with 10 nM DHT for 12 hrs and RNA is isolated and processed for expression microarrays. (Only for Reference)
Molecular Weight 470.51
Formula C23H21F3N6S
CAS No. 1628316-74-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 87 mg/mL (184.9 mM)

DMSO: 87 mg/mL (184.9 mM)

TargetMolReferences and Literature

1. Malik R, et al. Nat Med. 2015, 21(4):344-352.

Related compound libraries

This product is contained In the following compound libraries:
Apoptosis Compound Library Target-Focused Phenotypic Screening Library Transcription Factor-Targeted Compound Library NO PAINS Compound Library Anti-Aging Compound Library Inhibitor Library Histone Modification Compound Library Anti-Prostate Cancer Compound Library Anti-Cancer Compound Library Epigenetics Compound Library

Related Products

Related compounds with same targets
Sinapinic Acid JNJ-7706621 Ro 41-5253 Astragalin Ziyuglycoside I Curzerene Euscaphic acid NVP-2

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MI-136 1628316-74-4 Apoptosis Chromatin/Epigenetic Endocrinology/Hormones Epigenetic Reader Domain Androgen Receptor PPI inhibit MI 136 Inhibitor CRPC castration MI136 cancer resistant prostate inhibitor

 

TargetMol