Tyrosine Kinase/Adaptors PYK2 PF-562271


Catalog No. T2465   CAS 717907-75-0
Synonyms: PF562271, PF 562271,
Purity 100.00% Datasheet MSDS

PF-562271 is an effective ATP-competitive, reversible inhibitor of FAK(IC50=1.5 nM) and Pyk2 kinase(IC50=13 nM).

PF-562271, CAS 717907-75-0
Pack Size Availability Price/USD Quantity
1 mg In stock 68.00
2 mg In stock 89.00
5 mg In stock 134.00
10 mg In stock 167.00
25 mg In stock 256.00
50 mg In stock 410.00
100 mg In stock 533.00
1 mL * 10 mM (in DMSO) In stock 149.00
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Biological Description
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Description PF-562271 is an effective ATP-competitive, reversible inhibitor of FAK(IC50=1.5 nM) and Pyk2 kinase(IC50=13 nM).
Targets&IC50 CDK1/CyclinB :ic50 58nM,   CDK2/CyclinE :ic50 30nM,   CDK3/CyclinE :ic50 47nM,   FAK :ic50 1.5nM,   PYK2 :ic50 13nM,  
Kinase Assay The purified-activated FAK kinase domain (amino acid 410-689) is reacted with 50 μM ATP and 10 μg per well of a random peptide polymer of Glu and Tyr, p(Glu/Tyr), in kinase buffer (50 mM HEPES pH 7.5, 125 mM NaCl, and 48 mM MgCl2) for 15 min. Phosphorylation of p(Glu/Tyr) is challenged with serially diluted compound at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is tested in triplicate. Phosphorylation of p(Glu/Tyr) is detected with a general antiphospho-tyrosine (PY20) antibody followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody. HRP substrate is added, and absorbance readings at 450 nm are obtained after addition of stop solution (2 M H2SO4). IC50 values are determined using the Hill-Slope Model[1].
Cell Research
PF-562271 (Haoyuan Chemexpress Co., Ltd.) is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Ewing sarcoma cells are plated in 10-cm dishes, allowed to adhere for 24 hours, and then treated with PF-562271, PD0325901, or Dasatinib. ATP content is measured as a surrogate for cell number using the CellTiter-Glo Luminescent Cell Viability Assay. Luminescence readings are obtained using the FLUOstar Omega microplate reader. For experiments with small-molecule treatment, 1.25×103 Ewing sarcoma cells are seeded in each well and treated with a range of concentrations. IC50 values are calculated from ATP measurements obtained after 3 days of treatment using log-transformed, normalized data in GraphPad Prism 5.0. Cell lines are also treated with compound in 6-cm dishes, trypsinized, and counted by light microscopy using trypan blue exclusion. For experiments using shRNA-transduced cells, 1.25×103 cells are seeded per well into 384-well plates on day 3 posttransduction. ATP content is measured on days 3, 6, and 8 posttransduction[2].
Animal Research
Animal Model: Athymic femice bearing BxPc3 or PC3-M xenografts.
Synonyms PF562271, PF 562271,
Purity 100.00%
Appearance solid
Molecular Weight 507.49
Formula C21H20F3N7O3S
CAS No. 717907-75-0


-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 93 mg/mL (183.3 mM)

Ethanol: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% PEG400+0.5% Tween80+5% propylene glycol: 30 mg/mL


References and Literature
1. Roberts WG, et al. Cancer Res, 2008, 68(6), 1935-1944. 2. Lim ST, et al. Cell Cycle, 2008, 7(15), 2306-2314. 3. Canel M, et al. Cancer Res, 2010, 70(22), 9413-9422. 4. Sun H, et al. Cancer Biol Ther, 2010, 10(1), 38-43. 5. Bagi CM, et al. Cancer, 2008, 112(10), 2313-2321.

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Tyrosine kinase inhibitor library Kinase Inhibitor Library Anti-cancer Clinical Compound Library Fluorochemical Library Anti-Cardiovascular Disease Compound Library Angiogenesis related Compound Library Cell cycle related Compound Library

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