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SB-3CT

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Purity:99.38%

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COA HPLC HNMR

SB-3CT

Catalog No. T2009Cas No. 292605-14-2

SB-3CT is a potent and specific gelatinase inhibitor for MMP-2(Ki=13.9 nM) and MMP-9(Ki=600 nM).

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Pack Size	Price	Availability
1 mg	$41	In Stock
2 mg	$59	In Stock
5 mg	$90	In Stock
10 mg	$162	In Stock
25 mg	$259	In Stock
50 mg	$387	In Stock
100 mg	$576	In Stock
500 mg	$1,260	In Stock
1 mL x 10 mM (in DMSO)	$90	In Stock

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Product Introduction

Bioactivity

Description	SB-3CT is a potent and specific gelatinase inhibitor for MMP-2(Ki=13.9 nM) and MMP-9(Ki=600 nM).
In vitro	In a permanent cerebral ischemia mouse model induced by an embolus, SB-3CT protects neurons from ischemic cell death by inhibiting the degradation of neuronal adhesion proteins. Furthermore, daily intraperitoneal injections of SB-3CT (5-50 mg/kg) in the L-CI.5sT murine lymphoma model significantly hinders liver cancer cell metastasis. Likewise, in a model of osteolytic metastasis of human prostate cancer cells, daily intraperitoneal administration of SB-3CT (50 mg/kg) curtails cell growth, bone resorption, and angiogenesis.
In vivo	SB-3CT is capable of reducing osteolytic responses, aiding in the maintenance of bone integrity. In PC3 tumor cells, SB-3CT inhibits cellular growth. Within the basement membrane matrix, SB-3CT directly suppresses the infiltration of bone marrow endothelial cells and tubule formation.
Kinase Assay	kinase assays: PI3K inhibition by LY294002 is determined in a radiometric assay using purified, recombinant enzymes with 1?μM ATP. The kinase reaction is carried out for 1?hour at room temperature (24oC) and is terminated by addition of PBS. IC50 values are subsequently determined using a sigmoidal dose–response curve fit (variable slope). CK2 and GSK3β (glycogen synthase kinase 3β) inhibition is established by kinase selectivity screening. LY294002 is tested against the Upstate panel of kinases in 10?μM ATP.
Cell Research	SB-3CT is dissolved in DMSO (10 mM) and stored (-22°C), and then diluted with appropriate media (DMSO 1%) before use[2]. PC3 cells are seeded in 35-mm dishes (5×104 cells/dish) in complete culture medium. The next day, the medium is replaced with complete medium supplemented with 1% DMSO alone (vehicle) or SB-3CT (final concentrations 0.1-50 μM) in 1% DMSO. At various times, the cells are harvested with trypsin and counted[2].

Chemical Properties

Molecular Weight	306.4
Formula	C15H14O3S2
Cas No.	292605-14-2

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 6.1 mg/mL (20 mM)

DMSO: 55 mg/mL (179.5 mM)

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	3.2637 mL	16.3185 mL	32.6371 mL	163.1854 mL
5 mM	0.6527 mL	3.2637 mL	6.5274 mL	32.6371 mL
10 mM	0.3264 mL	1.6319 mL	3.2637 mL	16.3185 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1632 mL	0.8159 mL	1.6319 mL	8.1593 mL
50 mM	0.0653 mL	0.3264 mL	0.6527 mL	3.2637 mL
100 mM	0.0326 mL	0.1632 mL	0.3264 mL	1.6319 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc