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SB225002

Catalog No. T1955   CAS 182498-32-4

SB225002 is a potent and selective CXCR2 antagonist inhibiting interleukin IL-8 binding to CXCR2.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
SB225002 Chemical Structure
SB225002, CAS 182498-32-4
Pack Size Availability Price/USD Quantity
5 mg In stock $ 43.00
10 mg In stock $ 61.00
25 mg In stock $ 129.00
50 mg In stock $ 237.00
100 mg In stock $ 425.00
200 mg In stock $ 649.00
500 mg In stock $ 987.00
1 mL * 10 mM (in DMSO) In stock $ 48.00
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Purity: 98.79%
Purity: 98.39%
Purity: 96.86%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description SB225002 is a potent and selective CXCR2 antagonist inhibiting interleukin IL-8 binding to CXCR2.
Targets&IC50 CXCR2:22 nM
Kinase Assay Radioligand Binding Experiments: Assays are performed in 96-well microtiter plates where the reaction mixture contains 1.0 μg/ml membrane protein in 20 mM Bis-Tris-propane, pH 8.0, with 1.2 mM MgSO4, 0.1 mM EDTA, 25 mM NaCl, and 0.03% CHAPS and SB 225002 (10 mM stock in Me2SO) added at the indicated concentrations, the final Me2SO concentration is <1% under standard binding conditions. Binding is initiated by addition of 0.25 nM 125I-IL-8 (2,200 Ci/mmol). After 1-h incubation at room temperature the plate is harvested using a Tomtec 96-well harvester onto a glass fiber filtermat blocked with 1% polyethyleneimine, 0.5% BSA and washed three times with 25 mM NaCl, 10 mM Tris·HCl, 1 mM MgSO4, 0.5 mM EDTA, 0.03% CHAPS, pH 7.4. The filter is dried, sealed in a sample bag containing 10 ml of Wallac 205 Betaplate liquid scintillation fluid, and counted with a Wallac 1205 Betaplate liquid scintillation counter.
Cell Research Three esophageal squamous cell carcinoma cell lines WHCO1, WHCO5, and WHCO6 originally established from surgical biopsies of primary esophageal squamous cell carcinomas are cultured in DMEM containing 10% FCS at 37°C in a humidified atmosphere of 5% CO2. MTT assays are carried out using the Cell Proliferation kit I. Briefly, 1.5 × 103 cells are plated in 96-well plates in a final volume of 180 μL DMEM per well. SB 225002 (antagonist of CXCR2, 400 nM) is added to cells and 0.001% DMSO (solvent) is added as a control. After the indicated incubation period, 18 μL of the MTT labeling reagent (final concentration 0.5 mg/mL) is added to each well and incubated for 4 hours in a humidified atmosphere. One hundred eighty microliters of the solubilization solution are added to each well and the plates were left overnight at 37°C. The spectrophotometric absorbance of samples is measured at 595 nm using a microtiter plate reader.(Only for Reference)
Molecular Weight 352.14
Formula C13H10BrN3O4
CAS No. 182498-32-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 65 mg/mL (184.6 mM)

Ethanol: 3 mg/mL (8.51 mM), warmed

TargetMolReferences and Literature

1. White JR, et al. J Biol Chem. 1998, 273(17), 120095-120098. 2. Wang B, et al. Cancer Res. 2006, 66(6), 3071-3077. 3. Goda AE, et al. Biochem Pharmacol. 2013, 85(12), 1741-1752. 4. Sueoka H, et al. Surgery. 2014, 155(4), 640-649. 5. Manjavachi MN, et al. Eur J Pain. 2010, 14(1), 23-31. 6. Herz J, et al. Role of Neutrophils in Exacerbation of Brain Injury After Focal Cerebral Ischemia in Hyperlipidemic Mice. Stroke. 2015 Oct;46(10):2916-25. 7. Wang LY, et al. CXCL5 signaling is a shared pathway of neuroinflammation and blood-brain barrier injury contributing to white matter injury in the immature brain. J Neuroinflammation. 2016 Jan 6;13:6. 8. Shi ZR, et al. Decrease of galectin-3 in keratinocytes: A potential diagnostic marker and a critical contributor to the pathogenesis of psoriasis. J Autoimmun. 2018 May;89:30-40.

TargetMolCitations

1. Ji X, Sun T, Xie S, et al. Upregulation of CPNE7 in mesenchymal stromal cells promotes oral squamous cell carcinoma metastasis through the NF-κB pathway. Cell Death Discovery. 2021, 7(1): 1-11. 2. Wang W, Zhang M, Huang Z, et al. Knockdown of CXCL5 inhibits the invasion, metastasis and stemness of bladder cancer lung metastatic cells by downregulating CD44. Anti-Cancer Drugs. 2022, 33(1): e103-e112. 3. Wang W, Zhang M, Huang Z, et al. Knockdown of CXCL5 inhibits the invasion, metastasis and stemness of bladder cancer lung metastatic cells by downregulating CD44. Anti-Cancer Drugs. 2022, 33(1): e103-e112. 4. Ju C, Yuan F, Wang L, et al.Inhibition of CXCR2 enhances CNS remyelination via modulating PDE10A/cAMP signaling pathway.Neurobiology of Disease.2023: 105988. 5. Jiang S, Li W, Song M, et al.CXCL1-CXCR2 axis mediates inflammatory response after sciatic nerve injury by regulating macrophage infiltration.Molecular Immunology.2024, 169: 50-65.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Stem Cell Differentiation Compound Library ReFRAME Related Library Immuno-Oncology Compound Library NO PAINS Compound Library Cytokine Inhibitor Library Inhibitor Library Anti-Cancer Compound Library Chemokine Inhibitor Library Immunology/Inflammation Compound Library

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Keywords

SB225002 182498-32-4 Autophagy GPCR/G Protein Immunology/Inflammation CXCR SB-225002 CXC chemokine receptors Inhibitor inhibit SB 225002 inhibitor

 

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