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Azithromycin

Catalog No. T6401 CAS 83905-01-5

Synonyms: Zithromax, CP 62993, XZ-450

Azithromycin (CP 62993) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.

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Azithromycin, CAS 83905-01-5

Pack Size	Availability	Price/USD
50 mg	In stock	$ 56.00
100 mg	In stock	$ 84.00
500 mg	In stock	$ 219.00
1 mL * 10 mM (in DMSO)	In stock	$ 62.00

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Purity: 99.9%

Purity: 99.88%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Azithromycin (CP 62993) is an antibiotic by inhibiting protein synthesis, used for the treatment of bacterial infections.
In vitro	Azithromycin reduces about 40% of IL-8 mRNA and protein expression in cystic fibrosis (CF) cells reaching the levels of non-CF cells. Azithromycin results in 50% and 70% reduction of NF-kappaB and AP-1 DNA binding, respectively, leading to levels of non-CF cells. Azithromycin significantly enhances the intensity of a co-stimulatory molecule, CD80, on DCs but not CD86 and CD40 in dendritic cells (DCs). Azithromycin significantly increases the production of IL-10 and Clarithromycin (CAM) significantly inhibits the production of IL-6 by DCs. Azithromycin increases IL-10 and CAM decreases IL-2 productions significantly, when naive T cells derived from spleen are co-cultured with DCs treated in advance with LPS and these macrolides.Azithromycin selectively inhibits fluid-phase endocytosis of horseradish peroxidase and lucifer yellow in J774 mouse macrophages. Azithromycin delays sequestration of receptor-bound transferrin and peroxidase-anti-peroxidase immune complexes into cell-surface endocytic pits and vesicles. Azithromycin down-regulates cell surface transferrin receptors, but not Fc gamma receptors, by causing a major delay in the accessibility of internalized transferrin receptors to the recycling route, without slowing down subsequent efflux, resulting in redistribution of the surface pool to an intracellular pool.Azithromycin inserts into the DOPC lipid bilayer, so as to decrease its cohesion and to facilitate the merging of DPPC into the DOPC fluid matrix.
Cell Research	THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

Synonyms	Zithromax, CP 62993, XZ-450
Molecular Weight	748.98
Formula	C38H72N2O12
CAS No.	83905-01-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 74.9 mg/mL (100 mM)

Ethanol: 74.9 mg/mL (100 mM)

References and Literature

1. Cigana C, et al. P Biochem Biophys Res Commun, 2006, 350(4), 977-982. 2. Sugiyama K, et al. Clin Exp Immunol, 2007, 147(3), 540-546. 3. Tyteca D, et al. Exp Cell Res, 2002, 281(1), 86-100. 4. Fa N, et al. Biochim Biophys Acta, 2007, 1768(7), 1830-1838. 5. Menzel M, et al. Azithromycin augments rhinovirus-induced IFNβ via cytosolic MDA5 in experimental models of asthma exacerbation. Oncotarget. 2017 Mar 18. 6. Vandooren J, et al. Differential inhibition of activity, activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study. PLoS One. 2017 Apr 3;12(4):e0174853. 7. Fan Y, Li M, Ma K, et al. Dual-target MDM2/MDMX inhibitor increases the sensitization of doxorubicin and inhibits migration and invasion abilities of triple-negative breast cancer cells through activation of TAB1/TAK1/p38 MAPK pathway[J]. Cancer biology & therapy. 2019, 20(5): 617-632. 8. Xu K K, Huang Y K, Liu X, et al. Organotypic Culture of Mouse Meibomian Gland: A Novel Model to Study Meibomian Gland Dysfunction In Vitro[J]. Investigative Ophthalmology & Visual Science. 2020, 61(4): 30-30.

Citations

1. Nicholson M W, Huang C Y, Wang J Y, et al. Cardio-and Neurotoxicity of Selected Anti-COVID-19 Drugs. Pharmaceuticals. 2022, 15(6): 765 2. Xu K K, Huang Y K, Liu X, et al. Organotypic Culture of Mouse Meibomian Gland: A Novel Model to Study Meibomian Gland Dysfunction In Vitro. Investigative Ophthalmology & Visual Science. 2020, 61(4): 30-30 3. Fan Y, Li M, Ma K, et al. Dual-target MDM2/MDMX inhibitor increases the sensitization of doxorubicin and inhibits migration and invasion abilities of triple-negative breast cancer cells through activation of TAB1/TAK1/p38 MAPK pathway. Cancer biology & therapy. 2019, 20(5): 617-632. 4. Li H, Li J, Li J, et al.Carrimycin inhibits coronavirus replication by decreasing the efficiency of programmed–1 ribosomal frameshifting through directly binding to the RNA pseudoknot of viral frameshift-stimulatory element.Acta Pharmaceutica Sinica B.2024

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Microbial Natural Product Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Tumor Natural Product Library FDA-Approved Drug Library Anti-Infection Compound Library Covalent Inhibitor Library Human Metabolite Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Azithromycin 83905-01-5 Autophagy Microbiology/Virology Others Antibiotic Antibacterial CP62993 inhibit XZ 450 CP-62993 Bacterial XZ450 Zithromax CP 62993 XZ-450 Inhibitor inhibitor

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