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NH125

Catalog No. T6605 CAS 278603-08-0

NH125 is a selective eEF-2 kinase inhibitor with IC50 of 60 nM, <125-fold selectivity over PKC, PKA, and CaMKII, and also a potent histidine kinase inhibitor.

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NH125, CAS 278603-08-0

Pack Size	Availability	Price/USD
1 mg	In stock	$ 34.00
2 mg	In stock	$ 48.00
5 mg	In stock	$ 80.00
10 mg	In stock	$ 122.00
25 mg	In stock	$ 278.00
50 mg	In stock	$ 478.00
100 mg	In stock	$ 689.00
1 mL * 10 mM (in DMSO)	In stock	$ 89.00

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Description	NH125 is a selective eEF-2 kinase inhibitor with IC50 of 60 nM, >125-fold selectivity over PKC, PKA, and CaMKII, and also a potent histidine kinase inhibitor.
Targets&IC50	eEF2K:60 nM
In vitro	In C6 glioma cells, NH125 decreases the cellular content of phospho-eEF-2 without affecting total content eEF-2 content, and blocks cell cycle transit at the G1-S boundary. NH125 potently inhibits cell viability of 10 cancer cells with IC50 ranging from 0.7 to 4.8 μM. [1] NH125 effectively inhibits histidine protein kinases, including Envz, PhoQ, BvgS, EvgS, and thus produces potent anti-bacteria activities on oxacillin-resistant Staphylococcus aureus (ORSA), vancomycin-resistant Enterococcus faecalis (VRE), penicillin-resistant Streptococcus pneumoniae (PRS), and other Gram-positive and Gram-negative bacteria. [2] EEF2K inhibition by NH125 renders tumor cells more sensitive to curcumin and velcade, which possess ER stress-inducing action. [3]
In vivo	NH125 reduces blood pressure in SHR and ROS production, induction of inflammatory molecules, and hypertrophy in SHR superior mesenteric artery. [4]
Kinase Assay	eEF-2 Kinase Assay: eEF-2 kinase activity is measured by two methods: (a) a filter-based assay; and (b) by immunoblotting using antiphospho-eEF2 antibody. For both of these, reactions are carried out in 20 μl of total volume containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1.5 mM CaCl2, 100 μg/ml calmodulin, 2 μM His-tagged eEF-2 and 400 nM GST-eEF-2 kinase, and ATP mixture [50 μM ATP with 1μCi (γ-33P)ATP]. The kinase mixture without ATP is prepared on ice and then preincubated for 15 min at room temperature. Kinase reactions are started by adding ATP and allowed to progress at 30°C for 30 min. For the filter-based assay, the reaction is terminated by adding 20 μl of cold 1.5% phosphoric acid, and 5 μl of the reaction are applied to P81 Whatman phosphocellulose paper. The paper is washed three times in 500 ml of 0.5% phosphoric acid and once with 200 ml of acetone. The paper is then air-dried and immersed in 10 ml of scintillation mixture. Radioactivity is counted using a Beckton-Dickinson liquid scintillation counter. For immunoblotting, the reactions are stopped by addition of 20 μl of 3× Lamelli buffer [190 mM Tris (pH 6.8), 6% SDS, 30% glycerol, 15% 2-mercaptoethanol, and 0.003% bromphenol blue dye]. Samples are boiled for 5 min and resolved by 7% SDS-PAGE and processed for Western blotting as described below. Conditions for both assays are chosen to ensure linearity of the reaction with respect to time of incubation and concentration of enzyme.
Cell Research	The viability of cells is measured using an MTT assay. Briefly, 5 × 104 cells are plated in 96-well plates and exposed to various concentrations of drug for 48–72 h. The formazan product formed after 4 h incubation with MTT is dissolved in 100% DMSO and read at 550 nM using a Dynatech Microplate Reader MR5000. (Only for Reference)

Molecular Weight	524.56
Formula	C27H45IN2
CAS No.	278603-08-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 52.5 mg/mL (100 mM)

Ethanol: 39.3 mg/mL (75 mM)

References and Literature

1. Arora S, et al. Cancer Res. 2003, 63(20), 6894-6899. 2. Yamamoto K, et al. Biosci Biotechnol Biochem. 2000, 64(4), 919-923. 3. Cheng Y, et al. Autophagy. 2013, 9(2), 208-219. 4. Usui T, et al. Am J Physiol Heart Circ Physiol. 2013, 305(5), H756-768

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library Tyrosine Kinase Inhibitor Library TGF-beta/Smad Compound Library Anti-Neurodegenerative Disease Compound Library Highly Selective Inhibitor Library Anti-Fungal Compound Library Anti-Obesity Compound Library Bioactive Compounds Library Max Cytoskeletal Signaling Pathway Compound Library

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Calmodulin Kinase IINtide, Myristoylated W-9 hydrochloride Calmodulin-Dependent Protein Kinase II 290-309 acetate Rottlerin CALP2 acetate(261969-04-4 free base) KN-92 hydrochloride Psoralenoside CaMKII-IN-1

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Keywords

NH125 278603-08-0 Autophagy Chromatin/Epigenetic Cytoskeletal Signaling Microbiology/Virology Neuroscience Tyrosine Kinase/Adaptors Antibacterial PKA PKC Antifungal CaMK Virus Protease NH-125 NH 125 Calmodulin-dependent protein kinases Calmodulin-dependent kinases Inhibitor inhibit Fungal Bacterial inhibitor

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