Home Tools

TTNPB

Catalog No. T1288 CAS 71441-28-6

Synonyms: AGN191183, Arotinoid acid, Ro 13-7410,AGN-191183, Ro 13-7410

TTNPB (Ro 13-7410,AGN-191183), a potent RAR agonist, inhibits binding of [3H]tRA of human RARα (IC50: 5.1 nM), β (IC50: 4.5 nM), and γ (IC50: 9.3 nM), respectively.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

TTNPB, CAS 71441-28-6

Pack Size	Availability	Price/USD
2 mg	In stock	$ 33.00
5 mg	In stock	$ 52.00
10 mg	In stock	$ 79.00
25 mg	In stock	$ 165.00
50 mg	In stock	$ 323.00
100 mg	In stock	$ 488.00
500 mg	In stock	$ 1,060.00
1 mL * 10 mM (in DMSO)	In stock	$ 54.00

Bulk Inquiry

Select Batch

Purity: 98.84%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	TTNPB (Ro 13-7410,AGN-191183), a potent RAR agonist, inhibits binding of [3H]tRA of human RARα (IC50: 5.1 nM), β (IC50: 4.5 nM), and γ (IC50: 9.3 nM), respectively.
Targets&IC50	RARβ:4.5 nM, RARγ:9.3 nM, RARα:5.1 nM
In vitro	By inducing apoptosis, TTNPB (0.25 mg/kg) can inhibit the growth of the MXT-HI and MXT-HS models.
In vivo	After 72-hour cultivation in a conditioned medium, TTNPB increases the transcriptional activities of mouse mRARα (EC50: 2.0 nM), β (EC50: 1.1 nM), and γ (EC50: 0.8 nM) in JEG-3 cells. TTNPB exhibits high binding affinity to retinoic acid receptors, thereby inhibiting the binding of [3H]tRA to mRARα (IC50: 3.8 nM), β (IC50: 4.0 nM), and γ (IC50: 4.5 nM). TTNPB inhibits the growth of estrogen receptor-positive breast cancer cells and normal human mammary epithelial cells by inducing a G1 cell cycle arrest. Additionally, TTNPB concentration-dependently reduces the differentiation of ES-D3 cells.
Kinase Assay	Binding assays: Binding assays are performed as previously described (Allenby et al., 1993, 1994). Briefly, labeled and unlabeled retinoids are added to nucleosol or cytosolic fractions in ethanol so that the total amount of ethanol added is constant in all tubes and did not exceed 2% of the incubation volume. The receptor preparations are incubated with retinoids at 4°C for 4–6 hr. Sephadex PD-10 desalting columns are used to separate bound radioligand from free radioligand after equilib- rium is achieved. For competitive binding assays, varying concentrations of unlabeled competing ligand are incubated with the appropriate nucleosol or cytosol in the presence of a fixed concentration of [3H]tRA (sp. act. 49.3 Ci/mmol) or [3H]9-cis RA (sp. act. 24.0 Ci/mmol). Final concentrations of [3H] tRA and [3H]9-cis RA for nuclear receptor binding assays are 5 nM. Final concentrations of [3H] tRA for CRABP binding assays is 30 nM. The IC50s are calculated as described above (DeLean et al., 1978). For saturation kinetics, increasing concentrations of radiolabeled ligand ([3H]tRA sp. act. 49.3 Ci/mmol, [3H]TTNPB sp. act. 5.5 Ci/ mmol) are added to the nucleosol of the appropriate receptor subtype in the presence (nonspecific binding) or absence (total binding) of a 100-fold molar excess of the corresponding unlabeled retinoid. Specific binding is defined as the total binding minus nonspecific binding. Saturation kinetics are calculated as previously described (Scatchard, 1949; Grippo and Gudas, 1987; Levin et al., 1992).
Cell Research	Human mammary epithelial cells are maintained in Mammary Epithelial Basal Medium (MEBM) supplemented with the Mammary Epithelial Growth Media (MEGM) bullet kit. 184 and 184B5 cells are maintained in MEBM sodium-bicarbonate free (MEBM-SBF) supplemented with the MEGM bullet kit, isoproterenol (10 μM), and transferrin (5 μg/ml). MCF10A cell lines are maintained in DME/F12 containing 5% heat inactivated horse serum, penicillin/streptomycin (100 μg/ml and 100 μg/ml), hydrocortisone (1.4 μM), insulin (10 μg/ml), choleratoxin (100 ng/ml), and EGF (20 ng/ml). Breast cancer cell lines are maintained in Improved MEM Zinc Option containing 10% fetal bovine serum, 1% glutamine, and 1% penicillin/streptomycin. For growth assays, cells are treated with the different retinoids for the specified number of days with media and treatment changes every other day in T47D cells and every 2 days in 184 cells. Cell proliferation is measured according to the protocol for the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. This colorimetric assay determines the number of viable cells in a sample. Each point represents samples done in quadruplicate.(Only for Reference)

Synonyms	AGN191183, Arotinoid acid, Ro 13-7410,AGN-191183, Ro 13-7410
Molecular Weight	348.48
Formula	C24H28O2
CAS No.	71441-28-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 3.5 mg/mL (10 mM)

References and Literature

1. Pignatello MA, et al. Toxicol Appl Pharmacol. 1997, 142(2), 319-327. 2. Pignatello MA, et al. Toxicol Appl Pharmacol. 1999, 159(2), 109-116. 3. Wu K, et al. Breast Cancer Res Treat. 2006, 96(2), 147-157. 4. Louisse J, et al. Toxicol Lett. 2011, 203(1), 1-8. 5. Darro F, et al. Breast Cancer Res Treat. 1998, 51(1), 39-55.

Related compound libraries

This product is contained In the following compound libraries：

NO PAINS Compound Library Nuclear Receptor Compound Library Target-Focused Phenotypic Screening Library Stem Cell Differentiation Compound Library Lipid Metabolism Compound Library Bioactive Compound Library Neuronal Differentiation Compound Library Autophagy Compound Library Bioactive Compounds Library Max Metabolism Compound Library

Related Products

Related compounds with same targets

Coniferaldehyde Thonningianin A Apocynin dBET6 Barasertib Thioridazine hydrochloride Geniposidic Acid CM-272

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

TTNPB 71441-28-6 Apoptosis Autophagy Metabolism Retinoid Receptor Inhibitor Retinoic acid receptors RAR/RXR AGN191183 Arotinoid acid Ro 13-7410,AGN-191183 Ro 13-7410,AGN 191183 Retinoid X receptors Ro 13-7410,AGN191183 AGN 191183 Ro 13-7410 inhibit AGN-191183 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.