Home Tools

Valproic acid sodium salt

Catalog No. T1602 CAS 1069-66-5

Synonyms: Sodium Valproate

Valproic acid sodium salt (Sodium Valproate) is the sodium salt form of valproic acid with anti-epileptic activity. Valproic acid sodium salt is converted into its active form, valproate ion, in blood. Although the mechanism of action remains to be elucidated, Valproic acid sodium salt increases concentrations of gamma-aminobutyric acid (GABA) in the brain, probably due to inhibition of the enzymes responsible for the catabolism of GABA. This potentiates the synaptic actions of GABA. Valproic acid sodium salt may also affect potassium channels, thereby creating a direct membrane-stabilizing effect.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Valproic acid sodium salt Chemical Structure

Valproic acid sodium salt, CAS 1069-66-5

Pack Size	Availability	Price/USD
500 mg	In stock	$ 42.00
1 g	In stock	$ 50.00
5 g	In stock	$ 58.00
25 g	In stock	$ 66.00

Bulk Inquiry

Select Batch

Purity: 99%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Valproic acid sodium salt (Sodium Valproate) is the sodium salt form of valproic acid with anti-epileptic activity. Valproic acid sodium salt is converted into its active form, valproate ion, in blood. Although the mechanism of action remains to be elucidated, Valproic acid sodium salt increases concentrations of gamma-aminobutyric acid (GABA) in the brain, probably due to inhibition of the enzymes responsible for the catabolism of GABA. This potentiates the synaptic actions of GABA. Valproic acid sodium salt may also affect potassium channels, thereby creating a direct membrane-stabilizing effect.
Targets&IC50	HDAC1:0.4 mM
In vitro	In the MT-450 rat mammary carcinoma model, Valproic acid exhibits a delayed effect on the growth of primary tumors.
In vivo	In cultured cells, Valproic acid induces histone deacetylation similarly to the histone deacetylase inhibitor Trichostatin A, and like Trichostatin A, activates the transcription of various exogenous and endogenous promoters. However, in the embryos of vertebrates, both Valproic acid and Trichostatin A exhibit teratogenic effects without activating transcription. Valproic acid directly inhibits histone deacetylases through distinct pathways, with an IC50 of 0.4 mM for HDAC1. It inhibits cell proliferation or survival in F9 and P19 teratocarcinoma cells, as indicated by a decrease in [3H]thymidine incorporation, and promotes peroxisome proliferation in the liver of rodents. At a concentration of 1 mM, Valproic acid inhibits the release of Gal4 fused with N-COR, TR, or PPARδ in cells expressing the DNA binding domain of the glucocorticoid receptor and the ligand-binding domain of PPARδ, along with a GR-controlled reporter gene fusion. Valproic acid reduces the accumulation of acetylated histones and inhibits HDAC activity. Furthermore, Valproic acid induces specific types of differentiation, characterized by decreased proliferation, morphological changes, accumulation of the transcription factor AP-2, and expression of marker genes, where AP-2 serves as a potential marker for neuronal or neuroepithelial-like differentiation in F9 teratocarcinoma cells.
Kinase Assay	The activity of caspase-3, -8 and -9 is assessed using the caspase-3, -8 and -9 colorimetric assay kits, respectively. In brief, 1×106?cells in a 60-mm culture dish are incubated with 10 mM Valproic acid for 24 h. The cells are then washed in PBS and suspended in 5 volumes of lysis buffer provided with the kit. Protein concentrations are determined using the Bradford method. Supernatants containing 50 μg total protein are used to determine caspase-3, -8 and -9 activities. The supernatants are added to each well in 96-well microtiter plates with DEVD-pNA, IETD-pNA or LEHD-pNA as caspase-3, -8 and -9 substrates and the plates are incubated at 37°C for 1 h. The optical density of each well is measured at 405 nm using a microplate reader. The activity of caspase-3, -8 and -9 is expressed in arbitrary absorbance units.
Cell Research	Valproic acid is dissolved in DMSO. In brief, 5×105?cells are seeded in 96-well microtiter plates for MTT assays. After exposure to the designated doses of Valproic acid for the indicated times, MTT solution [20 mL: 2 mg/mL in phosphate-buffered saline (PBS)] is added to each well of the 96-well plates. The plates are additionally incubated for 3 h at 37°C. Medium is withdrawn from the plates by pipetting and 200 mL DMSO is added to each well to solubilize the formazan crystals. The optical density is measured at 570 nm using a microplate reader.

Synonyms	Sodium Valproate
Molecular Weight	166.2
Formula	C8H15NaO2
CAS No.	1069-66-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 8.3 mg/mL (50 mM)

H2O: 16.6 mg/mL (100 mM)

References and Literature

1. Phiel CJ, et al. J Biol Chem, 2001, 276(39), 36734-36741. 2. Göttlicher M, et al. EMBO J, 2001, 20(24), 6969-6978.

Citations

1. Bakar N A, Ibrahim W N W, Zulkiflli A R, et al.Embryonic mercury exposure in zebrafish: Alteration of metabolites and gene expression, related to visual and behavioral impairments.Ecotoxicology and Environmental Safety.2023, 256: 114862.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Neurodegenerative Disease Compound Library Anti-Cancer Approved Drug Library Inhibitor Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Membrane Protein-targeted Compound Library Anti-Cancer Active Compound Library Hematopoietic Toxicity Compound Library Anti-Viral Compound Library

Related Products

Related compounds with same targets

Naringin Sunitinib Malate GSK3-IN-3 Brefeldin A Betulinic acid Clioquinol Olaparib Parthenolide

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Valproic acid sodium salt 1069-66-5 Autophagy Chromatin/Epigenetic DNA Damage/DNA Repair Membrane transporter/Ion channel Microbiology/Virology Neuroscience Proteases/Proteasome Stem Cells Mitophagy GABA Receptor HIV Protease Gamma-secretase HDAC bipolar disorder Valproic acid anticonvulsant SCLC hepatic fat accumulation degradation Histone deacetylases HIV Mitochondrial Autophagy Notch inhibit Apoptosis Sodium Valproate anticancer Endogenous Metabolite headaches epilepsy small cell lung cancer Inhibitor Human immunodeficiency virus proteasomal Notch1 Valproic acid sodium migraine inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.