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Cytoskeletal Signaling Akt Vistusertib

Vistusertib

Catalog No. T1961   CAS 1009298-59-2
Synonyms: AZD2014

AZD2014 is an orally bioavailable inhibitor of the mammalian target of rapamycin (mTOR) with potential antineoplastic activity.

Vistusertib, CAS 1009298-59-2
Pack Size Availability Price/USD Quantity
2 mg In stock 63.00
5 mg In stock 80.00
10 mg In stock 120.00
25 mg In stock 216.00
50 mg In stock 300.00
100 mg In stock 480.00
1 mL * 10 mM (in DMSO) In stock 80.00
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Biological Description
Chemical Properties
Storage & Solubility Information
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Description AZD2014 is an orally bioavailable inhibitor of the mammalian target of rapamycin (mTOR) with potential antineoplastic activity.
Targets&IC50 mTOR :ic50 2.8nM,   P-Akt (S473) :ic50 80nM,   PI3Kα :ic50 3.766μM,   pS6 (S235/236) :ic50 200nM,  
Kinase Assay Recombinant truncated FLAG-tagged mTOR expressed in HEK 293 cells is used in biochemical assays, together with a biotinylated p70S6K peptide substrate. Streptavidin donor and protein A acceptor beads are used to assemble the capture complex for generation of the assay signal. The activity of the lipid kinases, PI3K alpha, beta, delta, and gamma are measured using recombinant proteins and the lipid PIP2 as substrate. Assays for ATM and DNA-PK activity are performed. The mTOR cellular activity is measured in MDAMB468 cells, using an Acumen laser scanning cytometer to analyze the levels of phosphorylation of S6 (Ser235/236) and AKT (Ser473)[1].
Cell Research
AZD2014 is prepared in DMSO (10 mM) and stored under nitrogen, and then diluted with appropriate media before use[1]. Cells are plated in 96-well plates for the indicated time. For CellTiterGlo assays: CellTiterGlo is mixed with the cells. Cells are normalized to day 0 control and net growth is determined using the following formula: ((x−y)/(z−y))=net growth, where x=reading of treated sample at end of study, y=average reading on day 0, and z=reading of DMSO-treated sample at end of study. The concentration of DMSO does not exceed 0.03% for any experiment. For MTS assays: adherent cell lines are grown in 96-well plates. MTS reagent is added on day 0 and on day 3 post-AZD2014 addition. Suspension lines are assayed using the Alamar Blue reagent, 72 hours after AZD2014 addition[1].
Animal Research
Animal Model: SCID mice
Synonyms AZD2014
Purity 98.29%
Molecular Weight 462.54
Formula C25H30N6O3
CAS No. 1009298-59-2

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 36 mg/mL (77.8 mM)

Ethanol: <1 mg/mL

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% PEG400+0.5% Tween80+5% propylene glycol: 30 mg/mL

Citations

References and Literature
1. Sylvie M, et al, AACR Annual Meeting, 2012, Abst 917. 2. Guichard SM, et al. Mol Cancer Ther. 2015, 14(11):2508-18.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Autophagy Compound Library PI3K-AKT-mTOR Compound Library Kinase Inhibitor Library Anti-cancer Clinical Compound Library

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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.