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METHODS: Preparation of D-Luciferin potassium for in vitro bioluminescence analysis:
1. Prepare 200× D-Luciferin reserve solution (30 mg/mL) with sterile water and mix gently until all dissolved. It can be used immediately after preparation or stored at -20℃.
2. Prepare 1× D-Luciferin (150 µg/mL) working solution in preheated tissue culture medium.
3. Aspirate the medium from the cultured cells.
4. Add 1× D-Luciferin solution to the cells before imaging. Incubate the cells at 37°C for a short period of time before imaging to increase signal.

For in vivo experiments at higher doses, D-Luciferin potassium (T4139) or D-Luciferin Sodium (T19743) are recommended.

Cells are incubated in 96-well plates (5,000 cells/well) and allowed to adhere for 24 hours in 1× DMEM media containing 10% FCS. The media are replaced with fresh DMEM media containing 5% FCS with or without various concentrations of tamoxifen or OP for indicated time periods. Cell viability is tested using WST-1 cell proliferation assay.(Only for Reference)