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Pack Size | Price | Availability | Quantity |
---|---|---|---|
5 mg | $39 | In Stock | |
10 mg | $64 | In Stock | |
25 mg | $118 | In Stock | |
50 mg | $197 | In Stock | |
100 mg | $369 | In Stock | |
200 mg | $549 | In Stock | |
1 mL x 10 mM (in DMSO) | $70 | In Stock |
Description | TBPB is an allosteric M1 mAChR agonist. |
Kinase Assay | in vitro kinase assay: MELK recombinant protein (0.4 μg) is mixed with 5 μg of each substrate in 20 μL of kinase buffer containing 30 mM Tris-HCl (pH), 10 mM DTT, 40 mM NaF, 10 mM MgCl2, 0.1 mM EGTA with 50 μM cold-ATP and 10 Ci of [γ-32P]ATP for 30 min at 30 °C. The reaction is terminated by addition of SDS sample buffer and boiled for 5 min prior to SDS-PAGE. The gel is dried and autoradiographed with intensifying screens at room temperature. OTSSP167 (final concentration of 10 nM) is dissolved in DMSO and added to kinase buffer before the incubation. |
Molecular Weight | 404.55 |
Formula | C25H32N4O |
Cas No. | 634616-95-8 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||
Solubility Information | DMSO: 4.05 mg/mL (10 mM) | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
DMSO
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