Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PND-1186 (VS-4718) is a reversible and specific FAK inhibitor (IC50: 1.5 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 48.00 | |
10 mg | In stock | $ 78.00 | |
50 mg | In stock | $ 222.00 | |
100 mg | In stock | $ 322.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 54.00 |
Description | PND-1186 (VS-4718) is a reversible and specific FAK inhibitor (IC50: 1.5 nM). |
Targets&IC50 | FAK:1.5 nM |
Kinase Assay | In vitro kinase activity: GST-FAK in vitro kinase activity is measured and compared to His-tagged FAK 411–686 using the K-LISA screening kit and poly(Glu:Tyr) (4:1) copolymer as a substrate immobilized on microtiter plates. IC50 values are determined with various concentrations of test compounds in a buffer containing 50 μM ATP and 10 mM MnCl2, 50 mM HEPES (pH 7.5), 25 mM NaCl, 0.01% BSA, and 0.1 mM Na orthovanadate for 5 min at room temperature. Serial diluted compounds are tested in triplicate. Substrate phosphorylation is measured using horseradish peroxidase-conjugated anti-pTyr antibodies with spetrophotometric color quantitation. IC50 values are determined using the Hill-Slope Model. Kinase selectivity profiling is performed by using the KinaseProfiler service. |
Cell Research | For soft agar assays, 48-well plates are coated with a 1:4 mix of 2% agar (EM Science) in 0.2 mL growth media (bottom layer). 5×104 cells are plated per well (in triplicate) in a mixture of 0.3% agar in 0.2 mL growth media (top layer). After agar solidification, 0.2 mL growth media is added containing DMSO or PND-1186 (final concentration for 0.6 mL). In separate experiments, PND-1186 is added after 4 days. After 10 days, colonies are imaged in phase contrast, enumerated by counting 9 fields (3 fields per well), and total area determined using Image J. For all analyses, experimental points are performed in triplicate and repeated at least two times. (Only for Reference) |
Synonyms | PND1186, PND 1186, VS-4718, SR-2516 |
Molecular Weight | 501.5 |
Formula | C25H26F3N5O3 |
CAS No. | 1061353-68-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 23 mg/mL (45.9 mM)
You can also refer to dose conversion for different animals. More
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
PND-1186 1061353-68-1 Angiogenesis Apoptosis Cytoskeletal Signaling Tyrosine Kinase/Adaptors FAK tumor integrin PTK2 inhibit growth Focal adhesion kinase PND1186 PTK2 protein tyrosine kinase 2 PND 1186 VS-4718 VS4718 SR2516 SR 2516 survival Inhibitor SR-2516 cell VS 4718 inhibitor