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Q-VD-OPH

Catalog No. T0282 CAS 1135695-98-5

Synonyms: Quinoline-Val-Asp-Difluorophenoxymethylketone

Q-VD-OPH (Quinoline-Val-Asp-Difluorophenoxymethylketone) is a pan-caspase inhibitor with potent antiapoptotic properties.

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Q-VD-OPH, CAS 1135695-98-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 47.00
5 mg	In stock	$ 87.00
10 mg	In stock	$ 147.00
25 mg	In stock	$ 237.00
50 mg	In stock	$ 368.00
100 mg	In stock	$ 547.00
1 mL * 10 mM (in DMSO)	In stock	$ 98.00

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Purity: 100%

Purity: 97.8%

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Description	Q-VD-OPH (Quinoline-Val-Asp-Difluorophenoxymethylketone) is a pan-caspase inhibitor with potent antiapoptotic properties.
Targets&IC50	Caspase-1:25 nM-400 nM, Caspase-8:25 nM-400 nM, Caspase-9:25 nM-400 nM, Caspase-3:25 nM-400 nM
In vitro	Q-VD-OPh (5-100 μM) potently inhibits Actinomycin D-induced DNA laddering and subsequent apoptosis with minimal toxicity in WEHI 231 cells. Q-VD-OPh prevents caspase mediated cleavage of PARP and activation of the major initiator and effector caspases. [2] Q-VD-OPh protects cardiac myocytes from virus-induced apoptosis in vitro. [4]
In vivo	Q-VD-OPh inhibits caspase-1 activity, IL-18 protein expression, and neutrophil infiltration during ischemic ARF in mice. [3] In vivo, caspase inhibition by Q-VD-OPh provides protection from virus-induced myocardial injury, with a significant reduction in caspase-3 activity. [4] In TgCRND8 mice, Q-VD-OPh inhibits caspase-7 activation and limits the pathological changes associated with tau, including caspase cleavage. [5]
Kinase Assay	Enzyme assay is conducted in buffer containing 25 mM Tris, pH 8.0, 1 mM DTT, 1 mM spermine, 50 mM KCl, 0.01% Nonidet P-40, and 1 mM MgCl2. PARP reaction contains 0.1 μCi [3H]NAD+ (200?000 DPM), 1.5 μM NAD+, 150 nM biotinylated NAD+, 1 μg/mL activated calf thymus, and 1?5 nM PARP-1. Autoreactions utilizing SPA bead-based detection are carried out in 50 μL volumes in white 96-well plates. Compounds (e.g., MK-4827) are prepared in 11-point serial dilution in 96-well plate, 5 μL/well in 5% DMSO/Water (10× concentrated). Reactions are initiated by adding first 35 μL of PARP-1 enzyme in buffer and incubating for 5 min at room temperature and then 10 μL of NAD+ and DNA substrate mixture. After 3 h at room temperature, these reactions are terminated by the addition of 50 μL of streptavidin-SPA beads (2.5 mg/mL in 200 mM EDTA, pH 8). After 5 min, they are counted using a TopCount microplate scintillation counter. IC50 data is determined from inhibition curves at various substrate concentrations[1].
Cell Research	Caspase inhibitors are added at the indicated concentrations 30 minutes prior to the addition of apoptotic stimuli. Viability and cell number are determined by trypan blue exclusion from three random fields of greater than 200 cells/field. All experiments are performed a minimum of three times.(Only for Reference)

Synonyms	Quinoline-Val-Asp-Difluorophenoxymethylketone
Molecular Weight	513.5
Formula	C26H25F2N3O6
CAS No.	1135695-98-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 100 mg/mL (194.75 mM)

DMSO: 50 mg/mL (97.37 mM)

References and Literature

1. Mischak R. ICN Bioconcepts. 2002, 8, 1–5. 2. Caserta TM, et al. Apoptosis. 2003, 8(4), 345-352. 3. Melnikov VY, et al. J Clin Invest. 2002, 110(8), 1083-1091. 4. DeBiasi RL, et al. J Virol. 2004, 78(20), 11040-11050. 5. Rohn TT, et al. Int J Clin Exp Med. 2009, 2(4), 300-308.

Citations

1. Wu Z, Lin C, Zhang F, et al.TIGD1 Function as a Potential Cuproptosis Regulator Following a Novel Cuproptosis-Related Gene Risk Signature in Colorectal Cancer.Cancers.2023, 15(8): 2286.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Neurodegenerative Disease Compound Library NO PAINS Compound Library Human Metabolite Library Protease Inhibitor Library Fluorochemical Library Anti-Cancer Compound Library Anti-Viral Compound Library Apoptosis Compound Library CNS-Penetrant Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Q-VD-OPH 1135695-98-5 Apoptosis Microbiology/Virology Proteases/Proteasome HIV Protease Caspase QVD-OPH QVDOPH inhibit Quinoline-Val-Asp-Difluorophenoxymethylketone Human immunodeficiency virus Inhibitor HIV Q VD OPH inhibitor

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