fluo 4 am

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).

Mag-Fluo-4 AM, a fluorescent Ca 2+ chelator, exhibits high affinity for calcium and is specifically designed to detect intracellular calcium ions. It features high sensitivity and low cytotoxicity. The compound's AM acetylmethyl ester form enhances cellular entry, and once inside, it is cleaved by intracellular esterases. This allows it to remain in the cell and bind to calcium ions, resulting in strong fluorescence [1].

IPG-4 AM, a membrane-permeable fluorescent indicator, specifically binds to potassium ions (K+) with a dissociation constant (Kd) of 7 mM. This compound exhibits excitation and emission maxima (λ) at 525 nm and 545 nm, respectively, and displays a 100:1 selectivity for K+ over sodium ions (Na+). It is utilized for real-time detection of intracellular K+ levels, as well as monitoring extracellular K+ changes in vivo.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-3FF AM is a low-affinity (Kd = 42 μM), Mg 2+ insensitive, and relatively photostable fluorescent Ca 2+ sensitive indicator (Abs Em = 462 nm 526 nm). It is essentially non-fluorescent but exhibits strong fluorescence enhancement upon entry into cells and binding to calcium. [1]