Antioxidant agent-3 (Compound 14q), a potent antioxidant, exhibits strong scavenging activity against DPPH radicals and ABTS+ radicals with IC50 values of 26.58 μM and 30.31 μM, respectively. Additionally, it enhances levels of reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH), while diminishing lactate dehydrogenase (LDH) in H2O2-treated HepG2 cells [1].

Antioxidant agent-3 (Compound 14q) demonstrates significant efficacy in scavenging DPPH and ABTS+ radicals with IC50 values of 26.58 μM and 30.31 μM, respectively. It exhibits low cytotoxicity towards human WI-38 (IC 50 > 100 μM) and GES (IC 50 > 200 μM) cells, indicating a favorable safety profile. Antioxidant agent-3 enhances viability and reduces apoptosis in H2O2-damaged HepG2 cells by decreasing ROS production and LDH release while improving GSH and SOD levels. These effects, coupled with its increased stability in methanol solution, underscore its potential as a therapeutic antioxidant. Assays, including cytotoxicity in WI-38 and GES cell lines and viability in H2O2-injured WI-38 and HepG2 cells, demonstrate reduced toxicity, enhanced cell viability, and protection against apoptosis and cellular damage.