fluorometric

Calcein (Fluorexon) is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage, a complexometric indicator for titration of calcium ions with EDTA, and for the fluorometric determination of calcium.

N-(9-Acridinyl)maleimide, a maleimide-type fluorescent thiol reagent, exhibits negligible inherent fluorescence; however, its conjugates with thiol compounds display pronounced blue fluorescence. This characteristic enables its use in the fluorometric analysis of cysteine and glutathione [1].

2,3-Diaminonaphthalene is a highly selective fluorometric and colorimetric reagent for selenium detection, and it is also used for the fluorometric determination of nitrite.

fluorometric substrate of cytochrome P450

fluorometric cytochrome P450 substrate

1,2-Diamino-3,4-ethylenedioxybenzene functions as a carbonyl-reactive fluorometric labeling agent.

Ac-YVAD-AFC is a fluorometric peptide substrate of caspase-1, featuring an excitation wavelength (λ excitation) of 400 nm and an emission wavelength (λ emission) of 505 nm [1] [2].

Resorufin benzyl ether is a fluorometric probe that acts as a substrate for cytochrome P450 (CYP)3A4. It is typically used near its apparent Km value of 30 μM to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Upon enzyme cleavage, resorufin benzyl ether generates a red fluorescent product (ex. 571 nm/em. 585 nm).

7-Ethoxyresorufin (Resorufin ethyl ether) (7-ER) is a fluorometric substrate and competitive inhibitor of cytochrome P450, particularly CYP1A1.