mca-devdapk(dnp)-oh

Mca-DEVDAPK(Dnp)-OH is a fluorescent substrate for caspase-3.

DNP-Pro-Leu-Ala-Leu-Trp-Ala-Arg-OH serves as a substrate for the matrix metalloproteinase MMP-1, exhibiting a Michaelis constant (Km) of 26.61 μM. Additionally, this compound is employed as a fluorescence probe for monitoring protein activity [1].

Mca-SEVNLDAEFK(Dnp) is a peptide FRET substrate for Beta-secretase 1 (BACE-1), featuring the 'Swedish' Lys-Met Asn-Leu mutation at the β-secretase cleavage site of the amyloid precursor protein (APP). Cleavage at the -Leu-Asp- site of Mca-SEVNLDAEFK(Dnp) releases the highly fluorescent 7-methoxycoumarin (Mca) fragment, which is freed from the quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher, leading to a significantly increased fluorescence intensity that is easily detectable.

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a quenched, fluorescent MMP peptide substrate designed for real-time quantification of MMP enzymatic activity. As an extended variant of the MMP substrate (FS-1), it demonstrates increased specificity against collagenases (MMP-1, MMP-8, MMP-13) and MT1-MMP, with higher specificity constants (Ex Em=325 nm 400 nm). [1]

Mca-RPPGFSAFK(Dnp)-OH, a fluorogenic substrate specifically designed for endothelin-converting enzyme-1 (ECE-1), enables the quantification of ECE-1 activity through its distinctive fluorescence signal. When ECE-1 cleaves this substrate, it releases 7-methoxycoumarin-4-acetyl (Mca), which exhibits excitation and emission maxima at 328 nm and 420 nm, respectively, allowing for the direct measurement of enzyme activity.

Mca-(Ala7,Lys(Dnp)9)-bradykinin is a fluorogenic substrate that demonstrates high sensitivity for ECE-1 (endothelin-converting enzyme-1). It features a (7-methoxycoumarin-4-yl)acetyl (Mca) group for fluorescence and a 2,4-dinitrophenyl (Dnp) group for quenching, yielding a significant increase in fluorescence when the substrate is cleaved [1].

Abz-SDK(Dnp)P-OH is a fluorescent peptide that functions as a substrate for the angiotensin I-converting enzyme (ACE). It features a fluorescent donor-acceptor pair comprising Abz and Dnp (2,4-dinitrophenyl) [1].

Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 is a fluorogenic substrate used for quantifying the activity of matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-26, thus facilitating the measurement of these enzymes' activity [1] [2].

Mca-AVLQSGFR-K(Dnp)-K-NH2 serves as a fluorogenic substrate specifically designed for the main protease (Mpro) of the severe acute respiratory syndrome coronavirus (SARS-CoV), also referred to as 3C-like protease (3CLpro). This substrate, upon enzymatic cleavage by SARS-CoV Mpro, liberates 7-methoxycoumarin-4-acetyl (Mca) which emits fluorescence. This fluorescence emission, with excitation/emission peaks at 328/420 nm respectively, facilitates the quantification of SARS-CoV Mpro's catalytic activity.

Mca-YVADAP-Lys(Dnp)-OH serves as a fluorogenic substrate specifically for caspase-1 and angiotensin-converting enzyme 2 (ACE2) [1].

Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 is a fluorogenic substrate designed for the quantification of β-secretase (BACE) activity. When cleaved by BACE, it liberates 7-methoxycoumarin-4-acetyl (Mca), a compound whose fluorescence, with excitation and emission peaks at 328 nm and 420 nm respectively, serves as a measurable indicator of BACE enzymatic action.

Mca-YVADAP-Lys(Dnp)-OH TFA is a fluorogenic substrate specifically used for caspase-1 and angiotensin-converting enzyme 2 (ACE2) [1].

Mca-SEVNLDAEFK(Dnp)-NH2 is a fluorogenic substrate featuring a 7-methoxycoumarin moiety whose fluorescence is quenched by the adjacent 2,4-dinitrophenyl group through resonance energy transfer. This compound serves as a probe for assessing peptidase activity; enzymatic cleavage of the amide bond separating the fluorophore and quencher results in increased fluorescence. It is particularly useful for quantifying the activity of BACE-1 [1].

Mca-KPLGL-Dap(Dnp)-AR-NH2 serves as a fluorogenic substrate specifically designed for matrix metalloproteinases (MMPs). This compound facilitates the quantification of MMP activity by releasing 7-methoxycoumarin-4-acetyl (Mca) upon enzymatic cleavage by MMPs, which exhibits fluorescence with excitation and emission maxima at 328 nm and 420 nm, respectively.

Mca-Ala-Pro-Lys(Dnp)-OH, a specific quenched fluorogenic substrate for ACE2, enables the detection of ACE2 activity in various tissues, including urinary, heart, and lung.