z-(l-arg)-amc

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

Boc-Gln-Ala-Arg-AMC hydrochloride is a fluorescent compound used as a substrate for trypsin and for assessing the protein hydrolysis activity of TMPRSS2.

Z-Gly-Pro-Arg-AMC hydrochloride is a fluorescent substrate used to assay the enzymatic activities of both trypsin and cathepsin K [1] [2].

Z-Gly-Pro-Arg-4MβNA is a thrombin substrate that, when cleaved by thrombin, releases 4-methoxy-2-naphthylamine (4MβNA). The liberated 4MβNA reacts with 5-nitrosalicylaldehyde, forming an insoluble yellow fluorescent product, which indicates the site of thrombin activity [1].

Z-Val-Gly-Arg-pNA, a chromogenic substrate for urokinase, can be used to determine urokinase activity [1].

Z-Gly-Pro-AMC serves as a fluorogenic substrate that, when hydrolyzed by prolyl endopeptidase, produces the highly fluorescent compound, 7-amido-4-methylcoumarin (λ ex =380 nm, λ em =465 nm) [1].

Z-Ala-Arg-Arg-AMC (Fluorogenic Proteasome Substrate) is a fluorogenic substrate utilized to assay trypsin-like activity within the proteasome [1].

Boc-Gln-Ala-Arg-AMC acetate is a fluorogenic substrate primarily used for assaying trypsin activity and is also suitable for evaluating the proteolytic function of TMPRSS2[1][2].

Boc-Leu-Ser-Thr-Arg-AMC serves as a fluorogenic substrate linked with AMC for proteases [1].

Z-Arg-Arg-4MβNA triacetate serves as a specific substrate for cathepsin B, producing the fluorescent product 4MβNA, which has an excitation wavelength (λ ex) of 355 nm and an emission wavelength (λ em) of 430 nm [1] [2].

Z-DEVD-AMC is a selective substrate for caspase-3, measurable through fluorescence spectrometry, and serves as a fluorescence reference standard for AMC-based enzyme substrates, including those for caspase [1].

Z-Val-Val-Arg-AMC serves as a fluorescent peptide substrate utilized for assessing the activity of cathepsins [1].