bioorthogonal

Itaconate-alkyne (ITalk) is a bioorthogonal probe for quantitative, site-specific chemoproteomic profiling of itaconate in inflammatory macrophages, facilitating biochemical evaluation and proteomic analysis of its direct targets.

Ac4GalNAlk, a weakly alkyne-labeled reagent for metabolic oligosaccharide engineering (MOE), facilitates the detection of protein glycosylation by promoting nucleotide-sugar biosynthesis and enhancing bioorthogonal cell surface markers. It is activated by cellular biosynthetic machinery into nucleotide sugars, subsequently enabling the tracing of glycoproteins in bioorthogonal chemistry [1].

Difluorinated H2S probe 1 is a fluorescent probe for hydrogen sulfide (H2S).1It selectively fluoresces in the presence of H2S over Zn2+, Fe3+, S2O32-, ClO-, SO32-, H2O2, NO2-, cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) when used at a concentration of 1 μM. Difluorinated H2S probe 1 displays excitation/emission maxima of 365/450 nm, respectively. 1.Zhang, J., Gao, Y., Kang, X., et al.o,o-Difluorination of aromatic azide yields a fast-response fluorescent probe for H2S detection and for improved bioorthogonal reactionsOrg. Biomol. Chem.15(19)4212-4217(2017)

Difluorocyclooctyne-CH2-COOH, a difluorinated cyclooctyne (DIFO) analogue, facilitates imaging of glycans on live cells by rapidly reacting with azides without requiring copper catalysis. This characteristic makes it an effective tool for bioorthogonal conjugation in live cell studies.

L-Homopropargylglycine is an alkyl chain-based PROTAC linker utilized in the synthesis of PROTACs[1].