thiol-groups

DTNB (Ellman’s Reagent) is used to quantify the number or concentration of thiol groups in a sample. It also can be used for measuring low-molecular-mass thiols such as glutathione in both pure solutions and biological samples.such as blood.

N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent that introduces thiol-groups into protein molecules and adds sulfhydryl groups in a protected form to proteins and other amine-containing molecules for later reaction with sulfhydryl reactive crosslinkers such as Sulfo-SMCC, Sulfo-MBS, etc.

ThioGlo1 is a thiol-reactive fluorescent probe.1,2,3Upon reaction with a thiol group, a fluorescent adduct is formed that displays excitation/emission maxima of 384/513 nm, respectively.2,3It also reacts with sulfite to form a fluorescent adduct with similar spectral characteristics, and interference from sulfite during thiol determination must be accounted for using secondary methods. ThioGlo1 has been used to monitor the oxidative stability of beer. 1.Yang, J.-R., and Langmuir, M.E.Synthesis and properties of a maleimide fluorescent thiol reagent derived from a naphthopyranoneJ. Heterocycl. Chem.28(5)1177-1180(1991) 2.Hoff, S., Larsen, F.S., Anderson, M.L., et al.Quantification of protein thiols using ThioGlo 1 fluorescent derivatives and HPLC separationAnalyst138(7)2096-2103(2013) 3.Lund, M.N., and Andersen, M.L.Detection of thiol groups in beer and their correlation with oxidative stabilityJ. Am. Soc. Brew. Chem.69(3)163-169(2011)

Biotin-PEG7-Maleimide is a biotinylation agent that selectively binds to thiol (SH) groups and is utilized in the synthesis of Drug-Linker Conjugates for Antibody-Drug Conjugates (ADCs) [1].

DM3 (Maytansinoid DM3) is a maytansine mimic containing disulfide or thiol groups and a tubulin inhibitor. It is the cytotoxic part of antibody-drug conjugates (ADCs) .

Thiol-PEG3-alcohol is a PEG derivative containing a hydroxyl group and a thiol group. The hydrophilic PEG spacer increases solubility in aqueous media. The hydroxyl group enables further derivatization or replacement with other reactive functional groups.

Maleimide-PEG4-NHS is a sulfhydryl and amine reactive heterofuncational PEG linker. The chemical bonds formed through Maleimide-PEG4-NHS linker are stable and are not cleavable. The NHS ester reacts with amino groups at pH 7-9 to form stable amide bond wh

Thiol-PEG2-acid is a PEG derivative containing a thiol group and a terminal carboxylic acid. The hydrophilic PEG spacer increases solubility in aqueous media. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. The terminal carboxylic acid can be reacted with primary amine groups in the presence of activators (e.g. EDC, or DCC) to form a stable amide bond.

Bis-PEG5-thiol is a PEG derivative containing two thiol groups. The thiol groups react with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. The hydrophilic PEG spacer increases solubility in aqueous media.