influenza matrix protein

Influenza A H1N1 (A Puerto Rico 8 34 Mount Sinai) Matrix protein 1 M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.3 kDa and the accession number is B4UPA8.

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.

Influenza A H3N2 (A Aichi 2 1968) Matrix protein 1 M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is I6SGW5.

Influenza A H1N1 (A Brevig Mission 1 1918) Matrix protein 1 M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is Q8BAC3.1.

Influenza A H7N9 (A Anhui 1 2013) Matrix protein 1 M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 28.5 kDa.

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.; Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1 XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Plays a major role in assembly, budding and uncoating of virion after membrane fusion. Completely covers the ribonucleoprotein coil and keep it in condensed bullet-shaped form. Inhibits viral transcription and stimulates replication. Plays a major role in early induction of TRAIL-mediated apoptosis in infected neurons. Rabies virus (RABV) (strain PM1503 AVO1) Matrix Protein (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 49.9 kDa and the accession number is P15200.

VSIV (strain Glasgow) Matrix Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 30.8 kDa and the accession number is P04876.

Influenza B (strain B Lee 1940) Nucleoprotein NP Protein (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 77.8 kDa and the accession number is P04665.

Influenza B (strain B Yamagata 1 1973) Nuclear export Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 18.4 kDa and the accession number is P08014.

Influenza A H3N2 (A Texas 50 2012) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.1 kDa and the accession number is R4L4F3.

Influenza A H10N8 (A duck Guangdong E1 2012) Neuraminidase NA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 50.2 kDa and the accession number is I6PU08.

Influenza A H5N1 (A chicken Jilin 9 2004) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 60.1 kDa and the accession number is Q692M2.

Influenza A H3N2 (A Sydney 5 1997) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.3 kDa and the accession number is C3PR59.

Influenza A H3N2 (A Hong Kong CUHK31987 2011) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.3 kDa and the accession number is L7S206.

Influenza A H1N9 (A mallard Ohio 265 1987) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 58.6 kDa and the accession number is A0M895.

Influenza B (B Massachusetts 03 2010) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.1 kDa and the accession number is L0HRV0.

Influenza A H1N1 (A USSR 90 1977) Hemagglutinin Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.3 kDa and the accession number is P03453.

Influenza B (B Massachusetts 03 2010) Hemagglutinin HA0 Protein is expressed in Baculovirus insect cells. The predicted molecular weight is 61.7 kDa and the accession number is L0HRV0.

Influenza A H1N1 (A swine Belgium 1 1998) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is C9WPQ9.

Influenza A H6N4 (A chicken Hong Kong 17 1977) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 52.6 kDa and the accession number is Q8UWX0.

Influenza A H3N2 (A X-31) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.1 kDa and the accession number is P03438.2.

Influenza A H3N8 (A equine Gansu 7 2008) Hemagglutinin HA1 Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 37.9 kDa and the accession number is C6ZEJ7.

Ebola virus EBOV (subtype Sudan, strain Gulu) VP40 Matrix protein VP40 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 34.5 kDa and the accession number is B0LPL6.

Influenza A H1N1 (A swine Belgium 1 1998) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.9 kDa and the accession number is C9WPQ9.

Influenza B (B Malaysia 2506 2004) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 60 kDa and the accession number is C0RVT0.

Influenza A H13N6 (A black-headed gull Sweden 1 1999) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 37.3 kDa and the accession number is Q5DL25.

Influenza A H7N8 (A mallard Netherlands 33 2006) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.5 kDa.

Influenza A H3N2 (A Indiana 07 2012) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.2 kDa and the accession number is J9T5Y9.

Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt GIN 2014 Kissidougou-C15) VP40 Matrix protein VP40 Protein (His & MBP) is expressed in E. coli expression system with His and MBP tag. The predicted molecular weight is 78.7 kDa and the accession number is X5H596.

Influenza A H7N9 (A Hangzhou 3 2013) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.6 kDa.

Influenza A H5N1 (A Egypt 3300-NAMRU3 2008) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is B6ECC1.

Influenza A H9N2 (A Hong Kong 35820 2009) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is D3G5Z1.

Influenza A H7N7 (A chicken Netherlands 1 03) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.4 kDa and the accession number is Q6VMK2.

Influenza A H1N1 (A Brisbane 59 2007) Hemagglutinin Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.7 kDa and the accession number is B0VX44.

Influenza A H2N2 (A Japan 305 1957) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is P03451.

Influenza A H3N2 (A Brisbane 10 2007) Hemagglutinin Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is A8W8K8.

Influenza A H1N1 (A California 04 2009) Hemagglutinin HA Protein ((IQS→RQRRRRK, HA1+HA2, partially cleaved,His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60 kDa and the accession number is C3W5S1.

Influenza A H5N1 (A Cambodia R0405050 2007) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is B6EBW8.

Influenza A H5N1 (A Hong Kong 483 1997) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.7 kDa and the accession number is O92930.

Influenza A H16N3 (A black-headed gull Sweden 5 99) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.5 kDa and the accession number is Q5DL21.

Influenza A H5N1 (A Vietnam 1194 2004) Hemagglutinin HA Protein (Native, HA1+HA2, cleaved, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60 kDa and the accession number is Q6DQ34.

Influenza A H4N6 (A Swine Ontario 01911-1 99) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is Q9E148.

Influenza A H1N1 (A New York 18 2009) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is C3W6C4.

Influenza A H10N3 (A duck Hong Kong 786 1979) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.7 kDa and the accession number is A2V811.

Influenza A H5N1 (A Japanese white-eye Hong Kong 1038 2006) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is A0FFY3.