flow cytometry

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.

6-CFDA N-succinimidyl ester (Carboxyfluorescein diacetate succinimidyl) is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.

GLF16 is a hydrophilic fluorescent SBB analog and robustly identifies senescence. It enables the use of flow cytometry and live cell sorting for rapid senescent cell detection. GLF16 is suitable for in vivo identification, isolation, and downstream characterization of senescent cell populations at the single-cell level in various biological materials.

GLF16 FA is a hydrophilic fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

BODIPY FL prazosin is a potent α1-adrenergic antagonist that inhibits both α1a-AR and α1b-AR. BODIPY FL prazosin is a fluorescent ligand (λEx (nm)/ λEm (nm): 485/535) that is often used in conjunction with flow cytometry or confocal laser scanning microscopy to study α1-adrenergic subtypes and cell imaging. cellular imaging.

Propidium Iodide (PI) is a red fluorescent dye that can be used for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of Propidium Iodide is 493/636 nm. And after binding with nucleic acid, the Ex/Em of PI-nucleic acid becomes 535/617 nm, while the fluorescence signal is enhanced 20-30 times.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry.

Fluo-3 and its derivative, Fluo3/AM, serve as fluorescent indicators for intracellular calcium (Ca2+), facilitating detection through flow cytometry and confocal laser scanning microscopy with visible light excitation (compatible with argon laser sources at 488 nm). Upon binding to calcium, these compounds exhibit a fluorescence intensity increase of approximately 40-fold.

eGFP circRNA-LNP, a lipid nanoparticle comprising eGFP circRNA, facilitates studies of RNA delivery, translation efficiency, and cell viability. This compound includes Enhanced Green Fluorescent Protein (eGFP) for expression within cells, detectable via fluorescence microscopy or flow cytometry [1].

Super Fluor 750 dye is a bright, near-IR–fluorescent dye with excitation ideally suited for the 633 nm laser line or dye-pumped laser excitation. Super Fluor 750 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in

Super Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Super Fluor 488 dye is pH-insensitive over a wide molar range.Probes with high fluo

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-3 (ammonium salt) is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. [1] Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

eGFP mRNA-LNP is a lipid nanoparticle encapsulating eGFP mRNA, designed for assessments of RNA delivery, translation efficiency, and cell viability. The eGFP mRNA, once internalized by cells, expresses Enhanced Green Fluorescent Protein (eGFP), facilitating detection through fluorescence microscopy or flow cytometry [1].

Fluo-3 (potassium salt) is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-3AM is a calcium indicator that exhibits an increase in fluorescence upon binding Ca2+. It is used to image the spatial dynamics of Ca2+ signaling in flow cytometry experiments.

Indo-1 (potassium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (potassium salt) binds calcium. Indo-1 (potassium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

Super Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Super Fluor 647 dye is pH-insensitive over a wide molar range.Probes

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentapotassium salt is most commonly used among the visible light-excitable calcium indicators.

Fluorescein di-beta-D-galactopyranoside (FDG) is a fluorescent substrate for the detection of β-galactosidase in kinetoplastids and animals by flow cytometry , λex=485 nm, λem=535 nm.

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

Indo-1 (sodium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (sodium salt) binds calcium. Indo-1 (sodium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

BODIPY 505/515 is a lipophilic fluorescent probe that localises to intracellular liposomes and has been used to label lipid droplets.BODIPY 505/515 has been used in electron microscopy, epifluorescence microscopy, confocal microscopy, and flow cytometry applications for various algal species.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

Methyl Green Zinc Chloride, a potent fluorescent dye, is utilized for staining DNA in cells and electrophoretic gels. It serves as a direct measure of viability through microscopy and flow cytometry, exhibiting peaks at 633 and 677 nm [1] [2]. For storage, it should be protected from light.

3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.

C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentaammonium salt is most commonly used among the visible light-excitable calcium indicators.

Super Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Super Fluor 680 dye is pH-insensitive over a wide molar range.The fluorescence q

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentasodium salt is most commonly used among the visible light-excitable calcium indicators.

PKH 26 is a red-fluorescent membrane dye, Ex/Em=551/567 nm.PKH 26 is commonly used in flow cytometry to measure T cell-mediated cytotoxic activity.

Fluo-3 is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

Methyl Green, a potent fluorescent dye, serves as a DNA stain for cells and electrophoretic gels. It facilitates direct measurement of viability via microscopy and flow cytometry, exhibiting peaks at 633 and 677 nm [1] [2]. For storage, it is imperative to protect Methyl Green from light.

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.