fluorescence microscopy

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex Em of PI is 493 636 nm. Upon binding with nucleic acid, the Ex Em shifts to 535 617 nm, enhancing the fluorescence signal 20-30 times.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentasodium salt is most commonly used among the visible light-excitable calcium indicators.

5-CFDA (5-Carboxyfluorescein diacetate)(5-Carboxyfluorescein diacetate) is membrane-permeant. It can be loaded into cells via incubation and hydrolyzed by intracellular esterases to 5-carboxyfluorescein that is used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.

FHZ is a fluorescent probe (IUPAC: 2-(7-hydroxy-3H-phenoxazin-3-one-10-yl)benzoic acid), widely utilized in biological imaging due to its high sensitivity and selectivity in detecting specific molecular targets within cellular environments, exhibiting optimal excitation and emission spectra for various fluorescence microscopy applications.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentaammonium salt is most commonly used among the visible light-excitable calcium indicators.

eGFP mRNA-LNP is a lipid nanoparticle encapsulating eGFP mRNA, designed to evaluate RNA delivery, translation efficiency, and cell viability. Upon cell internalization, it expresses Enhanced Green Fluorescent Protein (eGFP), enabling detection through fluorescence microscopy or flow cytometry [1].

Chol-N3 is a bioorthogonal chol probe capable of integrating with super-resolution fluorescence microscopy (SRM) to enable direct nanoscale imaging of lipid heterogeneity on resting living cell surfaces [1]. As a click chemistry reagent with an azide group, Chol-N3 undergoes copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-bearing molecules and can also participate in strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO or BCN group-containing molecules.

ATTO 425 NHS ester, a Rhodamine-structure-based fluorescent marker, exhibits strong absorption, high fluorescence quantum yield, and robust thermal and photochemical stability. This compound is ideal for applications in single molecule detection and high-resolution microscopy. As an NHS ester derivative of ATTO 425, it serves as a versatile labeling agent for proteins and antibodies.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentapotassium salt is most commonly used among the visible light-excitable calcium indicators.

BDP TR azide, a click chemistry reagent featuring an azide group, readily undergoes reactions with alkynes, DBCO, and BCN. Additionally, this compound functions as a fluorescent dye suitable for applications in fluorescence polarization assays and microscopy.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Acridine Orange Base, a cell-permeable fluorescent dye, selectively stains organisms (bacteria, parasites, viruses, etc.) bright orange and differentiates human cells in green under specific conditions (pH=3.5, Ex=460 nm), facilitating their detection via fluorescence microscopy. It exhibits green fluorescence upon binding to dsDNA (Ex=488, Em=520-524) and turns red with ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), making it useful in cell cycle assays [1] [2] [3].

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

eGFP circRNA-LNP, a lipid nanoparticle comprising eGFP circRNA, facilitates studies of RNA delivery, translation efficiency, and cell viability. This compound includes Enhanced Green Fluorescent Protein (eGFP) for expression within cells, detectable via fluorescence microscopy or flow cytometry [1].

Fluo-3 and its derivative, Fluo3/AM, serve as fluorescent indicators for intracellular calcium (Ca2+), facilitating detection through flow cytometry and confocal laser scanning microscopy with visible light excitation (compatible with argon laser sources at 488 nm). Upon binding to calcium, these compounds exhibit a fluorescence intensity increase of approximately 40-fold.

Bdp TR NHS Ester is a chemiluminescent coupling agent featuring an extended excited state lifetime, ideal for applications in immunoassays, microscopy, and fluorescence polarization analysis. This compound efficiently and specifically reacts with primary amine groups, including lysine residues or amino silane-coated surfaces, facilitating covalent bond formation under neutral or mildly basic conditions [1].

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Rhodamine B isothiocyanate (RITC) is a cell-permeant fluorogenic dye most often used as a conjugate to antibodies and proteins for fluorescence detection in microscopy. It displays excitation/emission maxima of 570/595 nm, respectively, and can be used in conjunction with immunohistochemistry for multi-labeling studies. It has also been used as an anterograde and retrograde tracer in neurons and in nanoparticles to detect apoptosis.