LAH4 acetate is the α-helical structure of the designed amphoteric peptide antibiotic, which is capable of complexing DNA, associating with the cell surface membrane and wrapped in the endosome, which is destroyed with the decrease of pH value. It has strong antibacterial, nucleic acid transfection and cell penetration activities. LAH4 acetate has high plasmid DNA transmission capacity. LAH4 acetate has a strong affinity for anions in bacterial membranes.

LAH4 TFA, an alpha-helix of the designed amphipathic peptide antibiotic, demonstrates potent antimicrobial, nucleic acid transfection, and cell penetration capabilities, alongside high plasmid DNA delivery capacities. It also exhibits a strong affinity for anionic lipids present in the outer membrane of bacterial membranes [1] [2] [3].

KALA, an amphiphilic peptide, adopts an α-helical conformation under physiological pH conditions. It functions as a fusogenic peptide, modifying the liposomal membrane that encapsulates plasmid DNA, thereby facilitating liver targeting and DNA transfection through galactose receptors.

The a-helix of the amphipathic peptide antibiotic LAH4 (KKALLALALHHLAHLALHLALALKKA-NH2) strongly interacts with phospholipid membranes, demonstrating in vitro transfection efficiencies comparable to those of commercially available reagents.