stain

1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.

SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

2,6-Diiodo-BODIPY 493 503, a fluorescent F-Bodipy dye, features a diiodo substitution at the (pyrrole) 2,6 position. This pyrrole iodine substituent imparts a red hue to the compound [1].

CelGreen nucleic acid gel stain *10,000× concentrate in water* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

1) Non-toxicity: The unique oiliness and high molecular weight characteristics of CelRed prevent it from penetrating cell membranes into cells. Ames test results also show that the mutagenicity of CelRed dye is much less than that of EB. 2) High sensitivi

Jenner's Stain, a dye utilized in microscopy, is employed for staining blood smears, chromosome staining via the C-banding technique, and routine blood examinations, including malarial staining [1].

1) Non-toxicity: The unique oiliness and high molecular weight characteristics of CelRed prevent it from penetrating cell membranes into cells, and Ames test results also show that the mutagenicity of CelRed dye is much less than EB. 2) High sensitivity:

Celfinder is a new kind of nucleic acid dye, which has the advantages of safety and sensitivity, and can be used as a dye for various kinds of nucleic acid electrophoresis.Unlike EB, which is highly carcinogenic, Celfinder is a flower-like dye with low to

Leishman's stain is a vital dye for staining peripheral blood and bone marrow smears, which appear pale bluish-grey to deep blue when viewed under an oil-immersion lens [1].

CelGreen nucleic acid gel stain *10,000× concentrate in DMSO* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

Giemsa stain, used in histology and microbiology, can stain chromatin and the nuclear membrane. It is employed in histopathology to detect malaria and other microorganisms, such as Histoplasma, Leishmania, Toxoplasma, and Pneumocystis [1].

Wright's stain is a useful organic compound for research related to life sciences and the catalog number is T65498.

BODIPY 493/503 (Pyrromethene 546) is a lipophilic fluorescent probe with Ex/Em of 493/503 nm. BODIPY 493/503 localizes to polar lipids and can be used to label cellular neutral lipid contents and for live and fixed cell applications.

Nucleic Acid Dye Green I, a cell-impermeable fluorescent dye, is widely utilized for quantifying both dsDNA and ssDNA due to its excitation and emission peaks at 497 nm and 520 nm, respectively. Additionally, it finds applications in gene expression and RT-PCR assays.

AMI5 (Eosin Y Disodium) is a versatile red dye used in cosmetics, pharmaceuticals, textiles, etc., and as tissue stain, vital stain, and counterstain with HEMATOXYLIN. It is also used in special culture media.

Neutral red is a vital dye, is used as an indicator and biological stain.

Light Green SF Yellowish (Acid Green 5) is an alkaline stain used for staining animal tissues and for cytoplasmic and fiber staining in plant histology.

Erythrosine B (Erythrosine I) , a tetraiodofluorescein, is served as a red coloring in some foods (cherries, fish), as a disclosure of dental plaque, and as a stain of some cell types. Its structural was similar to thyroxine.

Stains-All is a cationic dye of carbocyanine.

Acridine Orange hydrochloride is a fluorescent dye of cell-permeable. It binds to nucleic acids, resulting in an altered spectral emission.It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.

Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).

Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.

Toluidine Blue (Blutene chloride), a phenothiazine, is used as a biological stain, a hemostatic, and a dye for silk and wool. Blutene chloride is also used as a diagnostic aid for gastric neoplasms and oral, and used in the identification of the parathyroid gland in thyroid surgery.

Alizarin Cyanine Green F (NSC-41838) is an agent of green dye. It can be used to dye paper, chromed leather, wool, silk, and nylon (acid bath), to stain wood, and to color anodized aluminum, urea-melamine resin, and soap. It is used to color nylon 66 and or nylon 6 nonabsorbable surgical sutures for use in general surgery and used for coloring drugs generally, including drugs intended for use in the area of the eye. Alizarin Cyanine Green F is also proposed for use in semi-permanent hair dye formulations as a direct dye at a maximum concentration of 0.3% in the finished cosmetic product.

6-CFDA N-succinimidyl ester (Carboxyfluorescein diacetate succinimidyl) is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.

Hoechst 33342 (bisBenzimide H 33342), is a Benzimidazole fluorescent dye and a Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. HOE 33342 trihydrochlorde is used to quantify DNA in viable cells.

Hoechst 33258 analog 2 is part of a family of blue fluorescent dyes used to stain DNA.

Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA.

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. HOE 33187 is a cell dye for DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

3-Nitrobenzaldoxime serves as a stain utilized in biological research.

BODIPY493 503 methyl bromide, a BODIPY dye, is a small molecule with strong ultraviolet absorption, a sharp fluorescence peak, and high quantum yield. It exhibits relative insensitivity to environmental polarity and pH while maintaining stability under various physiological conditions. The structural asymmetry of BODIPY allows for diverse structural derivatives. BODIPY lipid droplet dyes effectively permeate the cell membrane, localize polar lipids, and specifically stain lipid droplets, making them suitable for labeling both live and fixed cells [1]. Maximum excitation emission wavelength: 493 503 nm [1].

Methyl Green, a potent fluorescent dye, serves as a DNA stain for cells and electrophoretic gels. It facilitates direct measurement of viability via microscopy and flow cytometry, exhibiting peaks at 633 and 677 nm [1] [2]. For storage, it is imperative to protect Methyl Green from light.

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. HOE 32021 is a cell dye for DNA. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of

AA-dUTP [sodium salt] is a fluorescent dye used for staining cDNA.

para-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation.

Crystal Ponceau 6R, a red azo dye, is utilized in histology for staining fibrin with the martius, scarlet, and blue (MSB) Trichrome stain [1].

DAPI (dilactate), a blue fluorescent dye, primarily targets and binds to AT clusters within the minor groove of dsDNA, significantly enhancing fluorescence by about 20-fold upon binding. It is particularly effective in staining the nucleus, allowing for clear identification of the cell cycle phases, while it does not stain the cytoplasm. The dilactate form of DAPI exhibits greater water solubility compared to its dihydrochloride counterpart. [1] [2]

ER-Tracker Green (ERTG) is a cell permeable live cell stain for live cell imaging.ER-Tracker Green has an excitation wavelength of 504 nm and an emission wavelength of 511 nm.

EthD-1 is a cell-impermeant viability indicator. It acts as a high-affinity nucleic acid stain.

Setoglaucine is a basic dye used to stain DNA.

Hoechst 33258 analog 6 is an analog of Hoechst stains which are part of a family of blue fluorescent dyes used to stain DNA.

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

MitoMark Green I is a green fluorescent mitochondrial stain [λmax ~ 490/516 nm].

Gallocyanine is a small molecule blue dye used to stain nuclei. It is known to inhibit Dickkopf-1 (Dkk1), an antagonist of the Wnt pathway. Gallocyanine can disrupt the interaction of Dkk1 with its receptor LRP5/6 (IC50 = 3 μM), thereby activating Wnt/β-catenin signaling. Modulation of the Dkk1-LRP5/6 interaction via gallocyanine has been shown to inhibit prostaglandin J2-induced tau phosphorylation at serine396 in primary cortical neurons in vitro.

Aniline Blue sodium, a water-soluble dye, serves as a biological stain for detecting nucleic acids and proteins in laboratory procedures like electrophoresis and microscopy. Its unique chemical properties enable it to bind to specific cellular components, causing a color change that aids in their visualization and analysis.

ortho-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation.

Biebrich scarlet is a scarlet dye, it has been used in Masson’s trichrome stain for the staining of collagen. It has also been used for the staining of eosinophil.