fluorescein

Fluorescein (Solvent Yellow 94) is a phthalic indicator dye, use as a synthetic fluorescent tracer.

Fluorescein octadecyl ester, a lipophilic fluorescent reagent, is immobilized in a plasticized PVC membrane for use in the reversible detection of alcohol molecules, enabling the determination of ethanol concentration in alcoholic beverages. Additionally, it serves as an acceptor in the fabrication of optrode membranes for picric acid quantification [1] [2].

Fluorescein hydrazide, a colorimetric probe synthesized through the reaction of dihydrazine adipate with fluorescein isothiocyanate, serves to label aldehyde and ketone groups [1] [2].

Fluorescein Di-β-D-Glucuronide, a fluorescent probe, enables non-invasive imaging of intestinal bacterial β-glucuronidase activity in nude mice. This compound's utilization for both in vitro and in vivo imaging offers valuable insights into bacterial β-glucuronidase activity, thereby enhancing pharmacodynamic research on its specific inhibitors in animal models [1].

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

Pentafluorobenzenesulfonyl fluorescein itself no fluorescence, but were in the H2O2 in sulfonyl key fluoresce when hydrolyzed. Pentafluorobenzenesulfonyl fluorescein is often treated as selective H2O2 sensors, can be used for the detection of H2O2 content in the cell.

Fluorescein Biotin is a biotin-substituting fluorescent dye that detects and quantifies biotin binding sites by fluorescence or absorbance.Fluorescein Biotin undergoes fluorescence quenching when bound to avidin or streptavidin.

Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).

Fluorescein-6-isothiocyanate is a useful organic compound for research related to life sciences. The catalog number is T64942 and the CAS number is 18861-78-4.

MTSEA-Fluorescein is a fluorescent probe that can be used for ion channel research [1] . Storage: protect from light .

Fluorescein di-beta-D-galactopyranoside (FDG) is a fluorescent substrate for the detection of β-galactosidase in kinetoplastids and animals by flow cytometry , λex=485 nm, λem=535 nm.

FITC (Fluorescein 5-isothiocyanate) is a derivative of fluorescein for the labeling of amines.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.

5(6)-Aminofluorescein (5(6)-AFM) is a high purity and quality chemical used as molecular probe and important as fluorescent agent.

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models.

FITC-Dextran (MW 60,000-76,000) is a fluorescein isothiocyanate (FITC)-labeled dextran fluorescent probe that is commonly used as a tracer molecule to label and track the action of cells or other biomaterials on semipermeable membranes.

5-Dodecanoylaminofluorescein, a lipophilic fluorescent probe, is conjugated with a free fatty acid derived from fluorescein. This compound is employed in analyzing membrane fluidity and assessing the critical micelle concentration of detergents. Additionally, it serves as a precursor in the synthesis of hydrophobic nanospheres suitable for drug delivery [1] [2] [3].

Dibenzylfluorescein (DBF), a fluorogenic probe (Fluorescent dye), serves as a substrate for various cytochrome P450 (CYP) isoforms, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). It is commonly utilized at its K_m value range of 0.87-1.9 µM (Ex=485nm, Em=535nm) to monitor alterations in CYP enzymatic activity attributable to pharmaceutical interventions or pathophysiological conditions [1] [2] [3] [4].

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.

6-Iodoacetamidofluorescein is a sulphhydryl-specific fluorescent dye utilized for selective labeling of -SH groups present in nuclear matrix polypeptides and proteins.

6-Aminofluorescein (6-AF) (6-AF) is a noval fluorescence marker.

Diiodofluorescein disodium salt is a dye.

4',5'-Dibromofluorescein (Eosinic acid) is a fluorescent dye that can be used as a ligand for protein characterization by spectroscopic analysis.

FITC-Dextran (MW 10000) (FITC-Dextran) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (10 kDa). FITC-Dextran can be used to determine ion, solute, and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used.

FAM hydrazide, 6-isomer, a fluorescein (FAM) derivative belonging to the xanthene dye family, serves as a labeling agent for carbonyl compounds, including aldehydes and ketones [1].

6-CR110, SE [6-Carboxyrhodamine 110, succinimidyl ester]*Single isomer* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

DiSBAC10, a voltage-sensitive fluorescent probe, facilitates cell membrane electrical activity investigation in FRET assays. At rest, it populates the membrane's outer leaflet, accepting photons from excited fluorescein-labeled proteins and emitting them at higher wavelengths. Upon cell depolarization, DiSBAC10 quickly translocates to the inner leaflet, increasing fluorophore separation and diminishing the FRET signal.

BDP FL maleimide, a thiol-reactive dye (Ex: 503 nm; Em: 509 nm), is utilized for protein labeling and peptide modification, serving as an alternative to fluorescein (FAM) in microscopy applications [1].

CAY10731 is a fluorescent probe for hydrogen sulfide (H2S).[1] Upon reaction with H2S, fluorescein (FITC) is released and its fluorescence can be used to detect H2S. FITC displays excitation/emission maxima of 485/535 nm, respectively. CAY10731 is selective for H2S over glutathione (GSH), cysteine, H2O3-, SO3-, and H2O2 as well as tBuO and OH radicals. It has been used to detect H2S in rat hippocampal slices and nematodes.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5'6-FAM-UUCUCCGAACGUGUCACGUTT, ACGUGACACGUUCGGAGAATT is a fluorescein (FAM)-labeled oligonucleotide, denoted by the sequence provided.

Cal Green 1 is a cell-impermeant fluorescent calcium indicator that is characterized by high quantum yield and low phototoxicity. Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green 1 appropriate for fluorescent microscopy. Cal Green 1 is ~5-fold brighter than fluo-3 at saturating calcium levels.

5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

Sulfane Sulfur Probe 4 (SSP4) serves as a fluorescent probe targeting sulfane sulfur species, encompassing hydrogen sulfide-derived polysulfides, hydropersulfides, and persulfides generated endogenously. Upon interacting with these species, SSP4 undergoes a nucleophilic attack, leading to fluorescein production, characterized by 494/515 nm excitation/emission peaks, along with the generation of two 1,2-benzodithiol-3-one molecules. This compound has been instrumental in localizing H2S3 within primary mouse neurons and identifying polysulfides in L. japonicus seedling roots.

6-CR110 [6-Carboxyrhodamine 110] *Single isomer* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

FA-alkyne (FA alkyne) is a fluorescein that is a competitive probe for natural S-succinylation in FH-/- and FH+/+ cells and can be used to synthesize nanophotosensors.

The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including neurodegeneration, aging, cancer, and infectious diseases.[1] There are a number of fluorescent reagents, such as 2,7-dichlorodihydrofluorescein (DCDHF), that can be used to detect free radicals, but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2). [2] HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence. [3] It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals.

BODIPY FL alkyne (BOD FL alkyne) is a green-fluorescent dye with an excitation maximum at 503 nm and an emission peak at 512 nm, exhibiting bright and photostable fluorescence within the fluorescein (FAM) channel. This fluorophore readily undergoes conjugation with various azide-containing molecules through copper-catalyzed Click Chemistry.

5-DTAF, an isomeric derivative of fluorescein, reacts directly with polysaccharides and alcohols in aqueous solutions at pH > 9. Its emission profile peaks at a wavelength of 518 nm following excitation at 488 nm [1].

ZnAF-1, a zinc sensor compound, utilizes a fluorescein-based structure incorporating the N,N-bis(2-pyridylmethyl)ethylenediamine chelating unit. Its primary function lies in the detection of Zn2+ ions. Through its binding capability, ZnAF-1 achieves a 1:1 stoichiometry with Zn(II).

APF is an aromatic amino-fluorescein derivative and fluorescent probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2), or other oxidants. Fluorescein displays excitation/emission maxima of 490/515 nm, respectively.

KMG-104AM is a selective fluorescein-derived magnesium fluorescent membrane-permeable probe. It is successfully used to Intracellular 3D Mg2+ Imaging.

Rhodamine 110 (RH110) is a xanthene dye similar to fluorescein and eosin.

Rose Bengal sodium is a potent inhibitor of VGlut and vesicular monoamine transporter (VMAT) (Ki of 19 and 64 nM for VGlut andVMAT, respectively）

AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein.

5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase.