4-di-16-asp

4-Di-16-ASP, a green fluorescent membrane dye, exhibits lipophilic properties and functions as an aminostyryl probe with a broad emission spectrum, detectable through green, orange, or red filters. This compound is frequently utilized in neuronal membrane tracing, offering quicker diffusion compared to DiO [1].

Di-4-ANEPPS, a voltage-sensitive dye, is utilized for detecting membrane potential changes, offering a time resolution under 1 ms and fluorescence variations up to 10% per 100 mV. Widely used for cardiac electrical activity mapping, Di-4-ANEPPS also shows a marked irreversible impact on both spontaneous heart rate and ventricular impulse conduction within an isolated rabbit heart model [1].

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

4-Di-1-ASP, a styryl dye, selectively stains glioma cells within live brain tissue, facilitating microscopic analysis of cell structure, viability, and processes including proliferation, endocytosis, cytokinesis, and phagocytosis. It also allows for the observation of mitochondrial structures in living cells. The dye exhibits green fluorescence when visualized under a microscope (λ ex /λ em = 475/606 nm) [1] [2].